广州地区乙肝病毒基因型分布及序列分析  

Sequence analysis and genotype distribution of hepatitis B virus in Guangzhou

作　　者：栾玉明[1] 刘达雄[1] 王国玲[1] 陆家海[2] 

机构地区：[1]广州市海珠区疾病预防控制中心,广东510288 [2]中山大学公共卫生学院

出　　处：《医学动物防制》2012年第10期1091-1092,1095,共3页Journal of Medical Pest Control

基　　金：海珠区科技计划项目(2007-Y-002)

摘　　要：目的对广州地区无症状携带者HBV基因型分布及变异情况进行初步分析,为其科学防制提供数据支持。方法测定65例广州地区无症状携带者HBV基因组全序列,应用DNAman软件构建系统发育树,确定基因型,并应用blastn进行各基因型同源性分析。结果 65例HBV无症状携带者中检测出B基因型50例(76.9%),C基因型14例(21.5%),D基因型1例(1.6%),广州地区以B基因型流行占优势。同源性分析结果表明,相同基因型间同源性高、稳定性好。结论广州地区HBV流行以B基因型为主,相同基因型的序列间同源性高、基因稳定性好。Objective To primarily analyze the HBV genotype distribution and variation of of asymptomatic carri- ers in Guangzhou, so as to provide data support for scientific control of HBV. Method Measure the complete sequence of HBV genome from 65 asymptomatic carriers in Guangzhou; determine genotypes by constructing phylogenetic tree with DNAman; and conduct homology analysis of each genotypes by blastn. Results Of the 65 HBV asymptomatic carriers, 50 cases were detected with genotype B （76.9%）, 14 cases with genotype C （21.5%） and 1 case with genotype D （ 1.6% ） . Genotype B was prevalent in Guangzhou. Homology analy- sis indicated that the same genotype had high homology and stability. Conclusions Genotype B of HBV was prevalent in Guangzhou. Sequence between same genotype had high homology and good genic stability.

关 键 词：乙型肝炎病毒 全基因组 基因型 

分 类 号：R373.21[医药卫生—病原生物学]