2种卷柏药材中穗花杉双黄酮和罗波斯塔-4′-甲醚含量测定研究  

Determination of amentoflavone and robustaflavone-4′-dimethyl ether in 2 species of herbs Selaginella

作　　者：邝俊维[1] 徐康平[1] 邹辉[1] 龙红萍[1,2] 喻凯[1] 李静[1] 权睿[1] 王慧[1] 谭桂山[1,3] 

机构地区：[1]中南大学药学院,长沙410013 [2]永州职业技术学院药学院系,湖南永州425100 [3]中南大学湘雅医院,长沙410008

出　　处：《中南药学》2012年第9期655-657,共3页Central South Pharmacy

基　　金：湖南省中医药科研计划(No.2009059);湖南省中医药科研计划(No.2010004);湖南省大学生创新训练(No.BY12076)

摘　　要：目的建立同时测定穗花杉双黄酮和罗波斯塔-4′-甲醚含量的方法,并在此基础上比较这2种成分在翠云草和卷柏中的含量差异。方法采用岛津VP-ODS色谱柱(4.6 mm×150 mm,5μm),流动相为乙腈-0.2%醋酸水溶液,梯度洗脱,流速1.0 mL·min-1,检测波长为338 nm,柱温为30℃。结果在此条件下,穗花杉双黄酮和罗波斯塔-4′-甲醚得到完全分离,均在0.600～12.00μg与峰面积线性关系良好(r=0.999 9),平均回收率分别为99.8%和97.7%,RSD分别为0.85%和1.1%(n=6)。结论该含量分析方法操作简单、快速、准确、灵敏度高、重现性好,为卷柏类药材中双黄酮类化合物含量测定提供参考。Objective To establish a method to determine the quantity of amentoflavone and robustaflavone-4′-dimethyl ether in Selaginella uncinata（Desv.） Spring and Selaginella.Methods The determination was performed on a Shimadzu VP-ODS column（4.6 mm×150 mm,5 μm）.Acetonitrile-0.2% acetic acid solution was used as the mobile phase with gradient elution.The flow rate was 1.0 mL · min-1.The UV detection wavelength was 338 nm.The column oven temperature was 30 ℃.Results Amentoflavone and robustaflavone-4′-dimethyl ether were separated,showing good linearity at 0.646-12.92 and 0.670-13.40 μg,respectively（r=0.999 9）.The average recoveries were 99.8% and 97.7%,and the RSDs were 0.85% and 1.1%（n=6）.Conclusion The method is accurate,simple and reproducible,and can be used to control the quality of herbs Selaginella

关 键 词：卷柏 翠云草 穗花杉双黄酮 罗波斯塔-4'-甲醚 含量测定 

分 类 号：R927.2[医药卫生—药学]