基于整体柱的超灵敏表面增强拉曼检测  

Ultra Sensitive Surface-Enhanced Raman Scattering Detection Based on Monolithic Column

作　　者：李青青[1] 杜一平[1] 唐慧容[1] 汪宣[1] 陈贵平[1] 王文明[1] 

机构地区：[1]华东理工大学上海市功能材料性化学重点实验室,上海200237

出　　处：《光散射学报》2012年第3期245-250,共6页The Journal of Light Scattering

基　　金：国家自然科学基金(20975039);国家基础研究计划(2007CB914100)

摘　　要：本文提出了一种全新的基于整体柱材料的SERS检测方法。通过将探针分子和银溶胶混合后滴加在整体柱上,我们可以得到浓度低至10-18 mol/L的罗丹明6G(R6G)及10-16 mol/L的对巯基苯胺(PATP)的SERS信号。利用原子力显微镜(AFM)和扫描电镜(SEM)对银溶胶及整体柱材料进行了表征。通过实验结果可以初步推测,整体柱材料的表面形态和孔结构可以促进银溶胶产生"热点"。A novel Surface-Enhanced Raman Scattering（SERS） detection method based on monolithic column which is commonly used in High Performance Liquid Chromatography（HPLC） was developed in the present study.The probe molecules were mixed with silver colloid before spread onto the monolithic column surface.In this study,SERS signals of Rhodamine 6G（R6G） at a concentration as low as 10-18 mol/L and p-aminothiophenol（PATP） at a concentration as low as 10-16 mol/L could be obtained through the use of monolithic column material.Atomic Force Microscope（AFM） and Scanning Electron Microscopy（SEM） were utilized to characterize the silver colloid and the monolithic column.It suggested that the surface morphology and the pore size do effectively assist the silver colloid in generating ＂hot spots＂.

关 键 词：表面增强拉曼光谱(SERS) 整体柱 超灵敏检测 罗丹明6G(R6G) 对巯基苯胺(PATP) 

分 类 号：O657.37[理学—分析化学]