离体肝脏低温保存—再灌注期间肝细胞凋亡现象观察  

OBSERVATION ON HEPATOCELLULAR APOPTOSIS DURING COLD PRESERVATION REPERFUSION OF ISOLATED LIVERS IN RABBITS

作　　者：汤礼军[1] 田伏洲[1] 王雨[1] 李晓军[1] 

机构地区：[1]成都军区总医院全军普外中心,成都610083

出　　处：《中国普外基础与临床杂志》2000年第4期214-216,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　探讨临床肝移植过程中离体供肝低温保存—再灌注损伤与肝细胞凋亡的关系。方法　采用细胞凋亡原位末端标记法并结合电镜观察 ,检测低温保存时间分别为 0、3、6、9小时的 4组兔肝脏在低温保存—再灌注过程中肝细胞凋亡发生情况。结果　在低温保存后的再灌注期间 ,4组动物的肝组织内均可见明显的肝实质细胞凋亡现象 ,而且低温保存时间越长的肝脏 ,其再灌注后产生的肝实质凋亡细胞数量越多 ;但各组肝脏于低温保存末 ,其组织内则未见或仅偶见肝实质细胞凋亡现象。Objective To find the relation between donor liver cold preservation reperfusion injury and hepatocellular apoptosis during liver transplantation. [WT5”HZ]Methods [WT5”BZ]Four groups of rabbit livers, which had experienced cold storage for 0,3,6,9hr respectively, were observed during their cold preservation reperfusion by using TdT mediated dUTP biotion nickend labeling and electron microscope. [WT5”HZ]Results [WT5”BZ]Apoptopic hepatic parenchymal cells were obviously observed in reperfusing livers subsequent to cold storage. Furthermore, the longer the cold storage duration, the greater the number of apoptotic cells. On the contrary, no or rare apoptotic hepatic parenchymal cells was observed in all the groups at the end of cold preservation. [WT5”HZ]Conclusion [WT5”BZ]It suggests that apoptosis of hepatic parenchymal cells is markedly involved in donor liver cold preservation reperfusion injury.

关 键 词：再灌注损伤 肝移植 离体供肝 低温保存 细胞凋亡 

分 类 号：R657.3[医药卫生—外科学]