TNFα对培养的脐静脉内皮细胞增殖的影响  

Effect of TNFα on endothelial cell proliferation

作　　者：董红梅[1] 喻伦银[2] 贾宗智[3] 张正彬[2] 张玉霞[2] 

机构地区：[1]汕头大学医学院病理学教研室,汕头515031 [2]湖北医科大学病理学教研室,武汉430071 [3]山西省长治医学院病理学教研室,长治046000

出　　处：《临床与实验病理学杂志》2000年第3期232-234,共3页Chinese Journal of Clinical and Experimental Pathology

摘　　要：目的 :探讨肿瘤坏死因子α(TNFα,4× 10 5U/L)对血管内皮细胞增殖的影响。方法 :将传代培养的脐静脉内皮细胞随机分为实验组和对照组两组 ,分别在细胞传代时加入含TNFα(TNFα含量为 4× 10 5U/L)和等量的不含TNFα的培养液 ,通过描绘生长曲线及运用图像分析测定DNA相对含量来观察两组细胞增殖情况。结果 :处理因素作用 3天内实验组细胞增殖快于对照组 ,而 3天后则慢于对照组。结论 :TNFα(4× 10 5U/L)对培养的脐静脉内皮细胞的增殖的影响具有双向性。Purpose To investigate the effect of TNFα on human umbilical vein endothelial cell proliferation.Methods Two groups were divided:experiment group,the medium with TNFα(4×10 5 U/L);control group,the medium without TNFα.TNFα was added in the cell passage. The growth curve and DNA image analysis were used for estimating the endothelial cell proliferation in the experiment. Results The cell proliferation was promoted in the first three days, but it was inhibited in the second three days. Conclusion TNFα has two sided effect on the proliferation of the umbilical vein endothelial cells.

关 键 词：肿瘤坏死因子Α 血管内皮细胞 细胞增殖 脐静脉 

分 类 号：R543.02[医药卫生—心血管疾病]