桂林地区乙肝患者HBVB基因亚型分布研究  

Investigation of HBV/B subgenotype distribution among patients with HBV in Guilin

作　　者：陈容娟[1] 梁伟秋[1] 杨媛[1] 黄城[1] 王慧敏[1] 朱广超[1] 刘永明[1] 苏何玲[1] 

机构地区：[1]桂林医学院生物技术学院,广西桂林541004

出　　处：《华夏医学》2012年第4期471-473,共3页Acta Medicinae Sinica

基　　金：桂林医学院项目驱动法课题资助;广西壮族自治区教育厅资助项目(200911MS167)

摘　　要：目的:研究桂林地区乙肝患者HBV B基因亚型分布特点,为本地区乙型肝炎的防治提供依据。方法:采用巢式PCR技术扩增乙肝患者HBV的S基因,经测序后与标准序列比对,采用Mega 5.0构建系统树进行基因亚型分型。结果:从120例乙肝病毒患者中鉴定出3个以前报告的乙肝病毒亚型,序列分型B2 104例,占86.7%;B4 1例,占0.8%;B5 1例,占0.8%,其中14例未分出亚型,占11.7%。结论:桂林地区HBV B基因亚型主要以B2为主,其次为B4、B5,未发现B1、B3、B6亚型。Objective： Distribution of HBV/B subgenotype among patients with HBV in Guilin was investigated,and provided basis for controlling and treatment of HBV.Methods： The S gene of hepatitis B virus was amplified by nest PCR and directly sequenced.The viral subgenotype was identified after phylogenetic analysis by Mega 5.0.Results： 3 previously reported HBV/B subgenotypes were identified from 120 patients.Sequence typing results showed：HBV/B2 was 104 cases,86.7%;HBV/B4 was 1 case,0.8%;HBV/B5 was 1 case,0.8%,and other subgenotypes’ such as HBV/B1,HBV/B3 and HBV/B6,were not found;subgenotypes were not known for 14 cases,11.7%.Conclusion： HBV/B2 is predominant among HBV infected patients in Guilin,the next one was HBV/B4 and HBV/B.HBV/B1,HBV/B3 and HBV/B6 were not found.

关 键 词：乙型肝炎病毒 S基因 B基因亚型 分布 

分 类 号：R512.6[医药卫生—内科学]