氨茶碱对哮喘大鼠气道炎症及重塑的影响  

Effect of aminophylline to astnma rats airway inflammation and remodeling

作　　者：张娟[1] 徐青[1] 安肃英[1] 

机构地区：[1]河北医科大学第二医院儿科,河北石家庄050000

出　　处：《临床荟萃》2012年第20期1776-1780,F0002,共6页Clinical Focus

基　　金：河北医科大学第二医院基金资助项目(2h0200915)

摘　　要：目的通过观察哮喘大鼠体内核转录因子κB(nuclear transcription factor-κB,NF-κB)、白细胞介素25(interleukin-25,IL-25)、基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)的变化,探讨氨茶碱治疗哮喘的作用机制。方法取30只Wistar雄性大鼠,随机分成正常对照组、哮喘模型组、氨茶碱治疗组各10只。采用卵白蛋白(ovalbumin,OVA)腹腔注射致敏加雾化吸入激发的方法制成哮喘模型,正常对照组大鼠用等量生理盐水取代OVA。氨茶碱治疗组在每次卵蛋白激发前30分钟给予氨茶碱60mg/kg腹腔注射。分别测定血清中IL-25,肺组织内NF-κB、MMP-9的表达水平。结果哮喘模型组、氨茶碱治疗组和正常对照组比较,血清IL-25、肺组织NF-κB和MMP-9依次降低,分别为血清IL-25(40.71±3.87)ng/L、(34.42±1.60)ng/L vs(25.69±1.74)ng/L,肺组织NF-κB0.34±0.03、0.26±0.04vs 0.16±0.03,肺组织MMP-9 0.28±0.02、0.23±0.02vs 0.17±0.01,各组间差异均有统计学意义(P<0.01)。肺组织内NF-κB与血清IL-25和MMP-9均呈显著正相关(r=0.795、0.881,均P<0.01)。结论①氨茶碱可明显抑制哮喘大鼠肺组织中NF-κB及血清中IL-25的水平,有效改善哮喘大鼠气道炎症状况。②氨茶碱可能通过降低肺组织中NF-κB、MMP-9的含量,起到改善气道重塑的作用。Objective To explore the mechanisms of aminophylline in asthma treatment by measuring nuclear transcription factor-κB（NF-κB）,interleukin-25（IL-25） and matrix metalloproteinase-9 （MMP-9） changes. Methods 30 Wistar male rats were randomly divided into normal control group（ n = 10）,asthmatic model group（ n = 10） and aminophylline treatment group （n = 10）. The rat model of asthma was established by intraperitoneal injection （sensitization） and nebulization of ovalbumin（OVA） （challenge）. Rats in normal control group were treated with normal saline instead of OVA. The rats of aminophylline treatment group were administered with aminophylline by intraperitoneal injection at a dose of 60 mg/kg before being stimulated by OVA in each time 30 minutes ago. All rats were sacrificed in the last 24 hours after the last challenge. IL-25 levels in serum and the expression of NF-κB and MMP 9 were detected. Results IL- 25, NF-KB and MMP-9 in pulmonary tissue were gradually decreased from asthmatic model group,aminophylline treatment group and normal control group ,respectively IL-25 （40.71 ±3.87） ng/L,（34. 42±1. 60） ng/L vs （25.69±1.74） ng/L,NF-KB 0. 34±0. 03,0. 26±0. 04 vs 0.16± 0. 03 , MMP-9 0.28± 0. 02,0. 23±0. 02 vs 0.17±0.01 ,the difference was statistically significant （ P 〈0.01 ）. The expression of NF-κB in pulmonary tissue and the concentration of IL-25 in serum and MMP-9 pulmonary tissue had a significant positive correlation（ r = 0. 795, P 〈0.01, r =0. 881, P 〈0.01）. Conclusion Aminophylline can inhibit the expression of NF- κB in pulmonary tissue and the concentration of IL-25 in serum of asthmatic rats, thus improve inflammatory state. Aminophylline can improve airway remodeling by reducing the expression of NF-κB and MMP-9 in pulmonary tissue.

关 键 词：哮喘 氨茶碱 NF-ΚB 白细胞介素25 基质金属蛋白-9 

分 类 号：R256.12[医药卫生—中医内科学]