氦-氧等离子体针灭活肺癌A549细胞  

Inactivation of A549 cancer cells by a helium-oxygen plasma needle

作　　者：陈维[1] 黄骏[1] 李辉[1] 吕国华[1] 王兴权[1] 张国平[1] 王鹏业[1] 杨思泽[1,2] 

机构地区：[1]中国科学院物理研究所软物质物理重点实验室,北京凝聚态物质物理国家实验室,北京100190 [2]厦门大学物理与机电工程学院,福建省等离子体与磁共振研究重点实验室,厦门361005

出　　处：《物理学报》2012年第18期320-324,共5页Acta Physica Sinica

基　　金：国家自然科学基金青年科学基金(批准号:11005151)资助的课题~~

摘　　要：研究了介质阻挡放电等离子体针对肺癌A549细胞的灭活机制,探讨从不锈钢管注入到氦等离子体尾流区域的氧气含量对杀灭肺癌细胞A549的影响.利用中性红吸收测试法定性观察了等离子体处理后死亡的细胞和活着的细胞的形态区别,并且定量测定了不同条件下的细胞存活率.在固定功率24 W的处理过程中,氦-氧等离子体的灭活效率主要取决于等离子体曝光时间以及氦气中添加氧气的百分含量.实验结果显示最好的处理参数为:处理时间150 s,800 mL/min的氦气添加3%氧气,保持针距样品的距离为3 mm.根据氦-氧等离子体发射光谱,可以推断在细胞灭活过程中,氦-氧等离子体中的活性粒子(如羟基和氧自由基)起主要作用.An inactivation mechanism of A549 cancer cells is studied by using a dielectric barrier discharge （DBD） plasma needle. The influence of oxygen concentration, which is injected into helium plasma afterglow region through a stainless steel tube, is investigated. The neutral red uptake assay provides a qualitative observation of morphological differences between the dead cells and the viable ceils after plasma treatment and a quantitative estimation of cell viability under different conditions. In the treatment process at a fixed power of 24 W, the inactivation efficiency of helium-oxygen plasma depends mainly on the exposure time and percentage of added oxygen in helium plasma. Experimental results show that the best parameters of the process are 150 s treatment time, 800 mL/min He with 3% Oz addition and separation of needle-to-sample 3 mm. According to the helium-oxygen emission spectra of the plasma jet, it is concluded that the reactive species （for example, OH and O） in the helium-oxygen plasma play a major role in the cell deactivation.

关 键 词：:氦-氧等离子体针 介质阻挡放电 A549癌细胞 发射光谱 

分 类 号：O539[理学—等离子体物理]