中心组合试验设计和响应面分析优化黄芩中黄芩苷提取工艺  

Optimization of the Extraction Technique of Baicalin Distilled from Scutellaria with Central Composite Design and Response Surface Method

作　　者：李思维[1] 谢蓉[1] 王梅[2] 郭东艳[1] 宋忠兴[1] 唐志书[1] 

机构地区：[1]陕西中医学院,陕西咸阳712046 [2]陕西中医学院附属医院,陕西咸阳712000

出　　处：《陕西中医学院学报》2012年第5期77-81,共5页Journal of Shaanxi College of Traditional Chinese Medicine

基　　金：国家十二五"重大新药创制"科技重大专项(2011ZX09401-308-035);教育部"新世纪优秀人才支持计划"项目(NCET-10-0931);陕西省科技统筹创新工程计划项目(2011KTCL03-05);陕西省教育厅产业化中试项目(2010JC05)

摘　　要：目的研究黄芩中黄芩苷的优化工艺条件,为进一步开发应用奠定基础。方法通过单因素实验考察了煎煮时间、加水倍量、煎煮次数3个因素对黄芩苷含量的影响,并在此基础上,通过Box-Behnken中心组合实验设计和响应面分析优化黄芩中黄芩苷提取工艺,以黄芩苷含量为评价指标进行分析。结果黄芩苷的最佳提取工艺条件为煎煮时间68.37 min,加水倍量10.46倍,煎煮3次,黄芩中黄芩苷含量较高,达到8.53%。结论获得的最佳提取工艺稳定可行,为进一步开发应用奠定基础。Objective To optimize extracting technology of baicalin distilled from scutellaria and lay an foundation for further development. Methods To observe the influence of the decocting time, the water addition and decocting frequency as the independent variables on baicalin content. Based on the observation, the experiment was optimized the extracting technology of baicalin distilled from scutellaria by Box - Behnken experimental design combined with response surface methodology. The response variable was the content of baicalin. Results The optimum extraction condition were to decoct for 2 times with 10.16 times of water and each time for 68.37 min, the content of baicalin is higher than other condition. The dried extraction yield was 8.53 %. Conclusion The experiment was get the best way to optimize the extraction technique, and laid the foundation for the further development and application.

关 键 词：黄芩苷 中心组合实验设计 响应面分析 

分 类 号：R284.2[医药卫生—中药学]