反相高效液相色谱法测定中药材虎杖中白藜芦醇含量  

RP-HPLC Determination of Resveratrol in TCM Polygonum Cuspidatum

作　　者：白祖海[1] 文刚[1] 张艳[1] 

机构地区：[1]长沙市疾病预防控制中心,长沙410001

出　　处：《理化检验（化学分册）》2012年第9期1045-1047,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：提出了反相高效液相色谱法测定虎杖中白藜芦醇含量的方法。以ODS-C18色谱柱(200mm×4.6mm,5μm)为分离柱,以乙醇-水(2+3)溶液作流动相淋洗,在紫外光检测波长304nm处进行测定。白藜芦醇的质量浓度在0.05～0.40mg.L-1范围内与峰面积呈线性关系,方法的检出限(3S/N)为10μg.L-1。方法用于中药材虎杖中白藜芦醇含量的测定,标准加入平均回收率为98.5%,测定值的相对标准偏差(n=5)为2.6%。RP-HPLC was applied to the determination of resveratrol in polygonum cuspidatum. The ODS- C18 column （200 min×4. 6 mm, 5 μm） was used as stationary phase, and a mixture of CH3CH2OH and H2O（2＋ 3） was used as mobile phase. The content of resveratrol was determined by UV-detection at 304 nm, Linear relationship between values of peak area and mass concentration of resveratrol was kept in the range of 0. 05- 0.40 mg·L-1 , with detection limit （3S/N） of 10μg· L-1. The proposed method was used in the determination of resveratrol in polygonum cuspidatum, giving values of average recovery obtained by standard addition method of 98.50% and RSD （n=5） of 2.6%.

关 键 词：反相高效液相色谱法 虎杖 白藜芦醇 

分 类 号：O652.63[理学—分析化学]