山东地区输血后丙肝患者HGV感染状况及HGV部分核酸序列测定  

HGV Infection in Post transfused Hepatitis C in Shandong Area and Sequencing of Partial HGV Gene

作　　者：于建国[1] 商庆华[1] 周秀梅[1] 王路[2] 赵平[2] 

机构地区：[1]解放军第88医院济南军区肝病研究所,271000 [2]第二军医大学

出　　处：《山东医药》2000年第12期4-6,共3页Shandong Medical Journal

摘　　要：应用 RT- PCR法检测庚肝病毒 ( HGV) RNA,并对阳性扩增的产物采用 PCR技术片段直接克隆法测定。结果从 86例输血后丙型肝炎 ( PTH- C)患者血清中检出 HGVRNA阳性者 31例 ( 36.0 % ) ,其中 1例 HGVNS5区部分核苷酸序列与美国原始株 ( u4 4 40 2 )和另 1例日本株 ( d872 55)核苷酸同源性比较分别为 86.0 %和 84 .0 %。证实山东地区 PTH- C患者存在着 HGV感染和 HGV/ HCV混合感染 ,但是否存在不同亚型或 HGVRNA阳性携带者 。To detect the HGV infection in Post transfused hepatitis C(PTH C)patients in Shandong and sequencing of partial HGV gene,RT PCR was performed to amplify the HGV gene from sera of PTH C patients One clone was sequenced from a PCR product Results showed that of 86 patients with PTH C,31 patients were HGVRNA positive(36 0%) One clone(358bp)from a patient with HGVRNA was sequenced Compared with other HGV isolates the homology of partial HGV gene of Shandong′s isolate,Was 86 0% with American isolates(u44402),84 0% with Japan isolate(d87255)respectively The conclusion is HGV infection exists in Shandong HGV and HCV confection are often seen The significance of genetic variation of HGV from different area and carriers remains to be determinated

关 键 词：输血后丙型肝炎 庚肝病毒 核苷酸序列 

分 类 号：R512.63[医药卫生—内科学]