不同治疗剂量的免疫抑制剂对大鼠生长发育的影响  

Study on the Effects of Different Therapeutic Doses of Immunosuppressive Agents on the Growth and Development of Rats

作　　者：陈林强[1] 何绿茵[1] 徐邦牢[2] 仉智[2] 林华欣[2] 李淼沅[2] 陈业辉[2] 

机构地区：[1]广州市第一人民医院检验科,广州510180 [2]广州市第一人民医院泌尿外科,广州510180

出　　处：《现代检验医学杂志》2012年第4期79-81,共3页Journal of Modern Laboratory Medicine

基　　金：广东省科技计划基金项目（编号：20098030801054）;广州市医药卫生科技重点项目基金（2008-zDi-07）.

摘　　要：目的观察不同治疗剂量的免疫抑制剂对大鼠生长发育的影响。方法建立不同治疗剂量的免疫抑制剂SD大鼠模型，按公式将肾移植术后环孢素A（CyclosporinA，CsA）、他克莫司（Tacrolimus，FK506）和雷帕霉素（Rapamycin，Ra—pa）的首剂治疗剂量换算成大鼠的治疗剂量，分别用25mg／kg／天，0．8mg／kg／天和2mg／kg／天胃饲作为药物干预组，生理盐水胃饲作为对照组，每组8只，胃饲8周。观察并比较各组大鼠生长发育及体重的变化情况。结果实验前各组大鼠体重无差异。建模8周后，环孢素A组大鼠明显消瘦、纳差、易激惹、毛发稀疏。对照组、环孢素A（CsA）组、他克莫司组（FK506）和雷帕霉素组（Rapa）大鼠体重分别为339．62±11．97g，296．50±22．69g，335．30±17．51g及342．56±15．29g；体重增长值分别为158．75±15．68，112．24±20．16，154．78±11．32g及160．91±13．51g。环孢素A（CsA）组大鼠体重增长值明显低于对照组，差异有统计学意义（P〈0.05）；他克莫司（FK506）组及雷帕霉素（Rapa）组大鼠体重增长值与对照组比较，差异无统计学意义（P〉0．05）。结论环孢素A（CsA）对大鼠生长发育的影响较明显；他克莫司（FK506）及雷帕霉素（Rapa）对大鼠的生长发育影响不明显。Objective To observe the effects of different therapeutic doses of immunosuppressive agents on rat growth. Meth- ods To establish different therapeutic doses of immunosuppressive agents in SD rat model,after renal transplantation,the first agent treatment doses of eyclosporine A （Cyclosporin A, CsA） （Taerolimus,FKS06）, tacrolimus and rapamycin （Rapa- mycin,Rapa） were converted into the therapeutic dose of rats, respectively, by the formula. 25 mg/kg/d,0. 8 mg/kg/d and 2 mg/kg/d gastric feeding were set as drug intervention group, saline gastric feeding as the control group, 8 rats in each group. The rats were fed by the gastric feeding for 8 weeks. Each rat growth and body weight changes were observed. Results There were no significant difference of the rats weight before the experiment began. 8 weeks after modeling, cyclosporin A treated rats showed marked weight loss,anorexia,irritable, sparse hair. The control group, cyclosporine A （CsA） （FK506）, tacrolimus and rapamycin （Rapa） treated rats showed increased weight, respectively, 339. 62±11.97 g, 296.50±22. 69 g, 335.30±17. 51 g and 342.56 ± 15.29 g; weight gain were 158.75 ±15.68 g, 112. 24± 20. 16 g, 154. 78 ±11.32 g, and 160. 91±13. 51g, respectively. Cyclosporin A （CsA） treated rats bodyweight growth was significantly lower than that of the control group （P〈0. 05）;（FK506） and rapamycin （Rapa） group of tacrolimus showed no significant difference of weight gain as compared with the control group （P〉0. 05）. Conclusion cyclosporin A （CsA） affected the growth of rat more sig- nificantly but FKS06 and Rapa had no significant effect on the rats weight.

关 键 词：免疫抑制剂 大鼠 生长发育 

分 类 号：R-332[医药卫生]