Fabricating a reactive surface on the fibroin film by a room-temperature plasma jet array for biomolecule immobilization  

作　　者：陈光良 郑旭 吕国华 张朝霞 Sylvain Massey Wilson Smith Michael Tatoulian 杨思泽 

机构地区：[1]Key Laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education,and Engineering Research Center for Eco-Dyeing&Finishing of Textiles,Zhejiang Sci-Tech University [2]Key Laboratory of Soft Matter Physics,Institute of Physics,Chinese Academy of Sciences [3]cole Nationale Suprieure de Chimie de Paris-Chimie ParisTech,Laboratoire de G'enie des Proc'ed'es Plasmas et Traitements de Surfaces,Universit'e Pierre et Marie Curie,Paris 75005,France

出　　处：《Chinese Physics B》2012年第10期313-320,共8页中国物理B（英文版）

基　　金：Project supported by the National Natural Science Foundation of China (Grant No. 11175157);the Young Scientists Fund ofthe National Natural Science Foundation of China (Grant No. 11005151);the Natural Science Foundation of Zhejiang Province,China (Grant No. Y6100045);the Project for Zhejiang Provincial Key Innovation Team,China (Grant No. 2012R10038)

摘　　要：A simple dielectric barrier discharge（DBD） jet array was designed with a liquid electrode and helium gas.The characteristics of the jet array discharge and the preliminary polymerization with acrylic acid（AA） monomer were presented.The plasma reactor can produce a cold jet array with a gas temperature lower than 315 K,using an applied discharge power between 6 W and 30 W（V dis × I dis）.A silk fibroin film（SFF） was modified using the jet array and AA monomer,and the treated SFF samples were characterized by atomic force microscopy（AFM）,scanning electron microscopy（SEM）,Fourier transform infrared spectroscopy（FTIR）,and contact angle（CA）.The deposition rate of the poly acrylic acid（PAA） was able to reach 300 nm/min,and the surface roughness and energy increased with the AA flow rate.The FTIR results indicate that the modified SFF had more carboxyl groups（-COOH） than the original SFF.This latter characteristic allowed the modified SFF to immobilize more quantities of antimicrobial peptide（AP,LL-37） which inhibited the Escherichia coli（E.Coli） effectively.A simple dielectric barrier discharge（DBD） jet array was designed with a liquid electrode and helium gas.The characteristics of the jet array discharge and the preliminary polymerization with acrylic acid（AA） monomer were presented.The plasma reactor can produce a cold jet array with a gas temperature lower than 315 K,using an applied discharge power between 6 W and 30 W（V dis × I dis）.A silk fibroin film（SFF） was modified using the jet array and AA monomer,and the treated SFF samples were characterized by atomic force microscopy（AFM）,scanning electron microscopy（SEM）,Fourier transform infrared spectroscopy（FTIR）,and contact angle（CA）.The deposition rate of the poly acrylic acid（PAA） was able to reach 300 nm/min,and the surface roughness and energy increased with the AA flow rate.The FTIR results indicate that the modified SFF had more carboxyl groups（-COOH） than the original SFF.This latter characteristic allowed the modified SFF to immobilize more quantities of antimicrobial peptide（AP,LL-37） which inhibited the Escherichia coli（E.Coli） effectively.

关 键 词：plasma jet array polymerizing modification fibroin film antimicrobial property 

分 类 号：O539[理学—等离子体物理] O358[理学—物理]