一过性离体大鼠肝脏灌流模型的建立  

Development of a Single-pass Isolated Rat Liver Perfusion Model

作　　者：徐乐加[1,2] 覃小玲[2] 王颖[2] 莫小兰[2] 毕惠嫦[2] 黄民[2] 陈孝[3] 

机构地区：[1]中山大学附属第三医院 [2]中山大学药学院临床药理研究所 [3]中山大学附属第一医院,广东广州510000

出　　处：《今日药学》2012年第8期449-451,463,共4页Pharmacy Today

基　　金：国家"重大新药创制"科技重大专项(编号:2009ZX09304-003)

摘　　要：目的利用UP-100通用灌流仪建立了一过性离体大鼠肝脏灌流模型。方法用Krebs-Henseleit碳酸氢盐缓冲液进行一过性离体大鼠肝脏灌流,在灌流时长90 min内通过检测AST、ALT和LDH的浓度,观察肝脏外观及胆汁流量,测定肝重变化,进行组织切片观察组织病理变化来评估离体灌流肝脏的损伤程度。结果 90 min灌流时长中,肝脏颜色质地正常,胆汁流量均匀,相应转氨酶释放量少,病理切片结果正常。结论离体大鼠肝脏在UP-100通用灌流仪90 min灌流过程中能保持较好的组织结构和肝脏功能,可用于离体肝脏的药物代谢研究。Objective To establish a single-pass isolated rat liver perfusion model in a Harvard UP-100 universal perfusion system. Methods The isolated rat liver was perfused using Krebs-Henseleit buffer in a single-pass manner in a Harvard UP-IO0 universal perfusion system. The functions of the liver were validated by examining the release of AST, ALT and LDH in perfusate, histological structures and the bile flow rate. Results During the 90 min perfusion, the apparent observation and the bile flow of the liver was normal, the release of AST, ALT in outlet perfusate was less than 10 U/L, while the LDH was less than 25 U/L, which meant the physiological functions of the rat livers was normal. The pathological examination showed that the histological structures the rat livers in this model were not changed. Conclusion The isolated rat liver model by using the universal perfusion system UP-100 is successfully developed, and can be applied to a drug metabolism study.

关 键 词：离体大鼠肝脏灌流 药物代谢 UP-100通用灌流仪 

分 类 号：R965.1[医药卫生—药理学]