白木香叶提取物对LPS诱导RAW264.7巨噬细胞炎症因子的影响  

Experimental Research of Aquilaria sinensis Peel Extract on RAW264.7 Cells Stimulated by LPS

作　　者：吴秀荣[1] 梅全喜[2] 林焕泽[1] 梁林源[1] 杨华[1] 杨丽华 蓝忠[1] 

机构地区：[1]广东省茂名市人民医院,广东茂名525000 [2]广州中医药大学附属中山医院,广东中山528401 [3]茂名市药品检验所,广东茂名525000

出　　处：《今日药学》2012年第8期471-473,共3页Pharmacy Today

基　　金：广东省中医药局科技项目(编号:20112060);茂名市科技计划立项项目(编号:2011068)

摘　　要：目的采用LPS刺激巨噬细胞RAW264.7,建立体外的炎症模型,探讨白木香叶提取物抗炎活性和作用机制。方法采用MTT法检测白木香叶提取物(ASPE)对RAW264.7细胞的毒性作用;采用ELISA法检测ASPE对IL-6表达情况;采用Western blotting检测iNOS和COX-2蛋白表达情况,结果 ASPE能抑制LPS刺激RAW264.7所产生的炎症反应,其作用机制可能与其抑制iNOS和COX-2蛋白表达有关。结论白木香叶提取物有抗炎活性。Objective To investigate the anti-inflammatory activity of Aquilaria sinensis peel extract （ASPE） on LPS- induced RAW264. 7 cells induced. Methods MTr assay was performed to determine the viability of ASPE on RAW264.7 ceils. ELISA was performed to establish the effects of ASPE on IL-6 production. The inhibitory effects of ASPE on the expression of iNOS and COX-2 were detected by Western blotting. Results ASPE could significantly suppressed inflammation in RAW264.7 ceils by LPS, and that could achieved by suppressing the protein level of iNOS and COX-2. Conclusion There is anti-inflammatory activity in Aquilaria sinensis peel extract.

关 键 词：白木香叶 提取物 抗炎活性 药理作用 

分 类 号：R965[医药卫生—药理学]