氨氮对凡纳滨对虾稚虾的急性毒性作用  

Acute Toxic Effects of Ammonia Nitrogen on Litopenaeus vannamei

作　　者：魏俊利 孙金生[1,2] 李翔 马文婷 耿绪云 徐赟霞 李晶晶 

机构地区：[1]天津市水生动物疫病预防控制中心,天津300221 [2]天津师范大学细胞和分子调控重点实验室,天津300387

出　　处：《天津农学院学报》2012年第3期58-61,共4页Journal of Tianjin Agricultural University

基　　金：国家重点基础研究发展计划(973计划)项目"对虾白斑综合征防治的免疫学途径和关键技术原理"(2012CB114405)

摘　　要：实验研究了氨氮对体长3.2 cm、体质量0.356 g(n=10)与体长1.6 cm、体质量0.036 g(n=15)两种规格凡纳滨对虾(Litopenaeus vannamei)稚虾的急性毒性效应。血氨和碱性磷酸酶活性的测定结果显示,血氨随氨氮浓度的升高而升高,碱性磷酸酶(AKP)活性随氨氮的升高而下降。急性毒性实验结果表明,体长3.2 cm稚虾的游离态氨24 h LC50为2.068 mg/L、48 h LC50为1.654 mg/L、96 h LC50为1.395 mg/L,生存安全浓度Sc为0.396 9 mg/L;体长1.6 cm稚虾的游离态氨24 h LC50为1.577mg/L、48 h LC50为1.042 mg/L、96 h LC50为1.012 mg/L,生存安全浓度Sc为0.206 5 mg/L。It is studied the acute toxic effects of ammonia nitrogen on two specifications of L. vannamei which mean body weights （MBW） were 0.356 g and 0.036 g, respectively. The concentrations of blood ammonia and activities of alkaline phosphatase （AKP） were analyzed. The results show that variation trend of concentrations of blood ammonia were consistent with the ammonia nitrogen, while the activities of alkaline phosphatase （AKP） were decreasing when the ammonia nitrogen raised. The median lethal concentrations （LCso） of NH3＋ on shrimp and the safe concentration （Sc） were also investigated. The LCso ofNH3＋ on shrimp with MBW 0.356 g were 2.068 mg/L at 24 h, 1.654 mg/L at 48 h and 1.395 mg/L at 96 h, while the LCso ofNH3＋ on shrimp with MBW 0.036 g were 1.577 mg/L, 1.042 mg/L and 1.012 rag/L, respectively. The Sc was 0.396 9 mg/L and 0.206 5 mg/L to shrimp with MBW 0.356 g and 0.036 g, respectively. It is concluded that the changes of ammonia nitrogen have certain influence on L. vannamei, and the ammonia nitrogen was one of the important factors for the health and the survival of L. vannamei.

关 键 词：凡纳滨对虾稚虾 碱性磷酸酶 血氨 游离态氨 半数致死浓度 生存安全浓度 

分 类 号：S912[农业科学—水产科学]