黄芪对急性肺损伤模型大鼠肺组织水通道蛋白-1和水通道蛋白-5表达的影响  

Effect about Astragalus mongholicus on AQP-1 and AQP-5 expression in lung tissue of ALI model rat

作　　者：刘毅[1] 梅荣[1] 杨明会[1] 李绍旦[1] 王文明[1] 

机构地区：[1]中国人民解放军总医院中医院,北京100853

出　　处：《环球中医药》2012年第9期651-653,共3页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(30873344)

摘　　要：目的探讨补气中药黄芪对急性肺损伤模型大鼠肺组织水通道蛋白-1(AQP-1)和水通道蛋白-5(AQP-5)表达的影响。方法将30只雄性SD大鼠随机分为正常对照组、模型组、中药组各10只,予以每ml相当于0.25g黄芪生药的煎剂干预后,使用凝胶电泳测条带积分光密度值来检测确定肺组织AQP-1和AQP-5的表达情况。结果中药组大鼠肺组织AQP-1的积分光密度值为(0.572±0.084),模型组大鼠肺组织AQP-1的积分光密度值为(0.278±0.068),中药组高于模型组(P<0.01)。中药组大鼠肺组织AQP-5的积分光密度值为(0.812±0.084),模型组大鼠肺组织AQP-5的积分光密度值为(0.525±0.045),中药组高于模型组(P<0.05)。中药组与正常对照组无明显差异(P>0.05)。结论黄芪可能是通过促进肺组织AQP-1、AQP-5的表达,从而起到对模型大鼠急性肺损伤的防治作用。Objective To investigate the effect of supplementing qi herbs astragalus mongholicus （huangqi） on AQP-1 and AQP-5 expression of lung tissue of ALI model rat induced by LPS. Methods 30 male Sprague Dawley rats were randomly divided into normal control group, model group, herbs group, each 10. Test AQP-lmRNA and AQP-SmRNA expression of lung tissue, after the supplementing qi herbs used. Results AQP-lmRNA IOD of herbs group （0. 572±0. 084）, AQP-lmRNA IOD of model group （0.278±0.068） , herbs group higher than model group （P〈0.01）; AQP-SmRNA IOD of herbs group （0. 812±0. 084）, AQP-SmRNA IOD of model group （0. 555±0. 045）, herbs group higher than model group （ P〈0.05 ） ; There were no statistical significance between herbs group and normal control group （ P〉 0.05 ）. Conclusion Supplementing qi herbs astragalus mongholicus prevent and treat the ALl probably by promoting the AQP-lmRNA and AQP-SmRNA expression of lung tissue.

关 键 词：急性肺损伤 黄芪 水通道蛋白-1 水通道蛋白-5 

分 类 号：R285.5[医药卫生—中药学]