三种血清微小RNA的检测在糖尿病肾病诊断中的价值评估  

Analysis on the level of three miRNAs in serum of patients with diabetic nephropathy

作　　者：曾健英[1] 何凤[2] 童俊容[2] 罗正茂[2] 张虹[2] 王寅[2] 黄远航[2] 刘宏[2] 张建林[2] 

机构地区：[1]广州军区广州总医院内六科,510010 [2]广州军区广州总医院肾内科,510010

出　　处：《新医学》2012年第8期531-533,共3页Journal of New Medicine

基　　金：国家自然科学基金(81170765);广东省科技计划项目(2011B061200034;2011B061300020)

摘　　要：目的:检测miR-21、miR-192和miR-200c 3种微小RNA(miRNA)在糖尿病肾病(DN)患者血清中的含量,评价其在DN诊断中的价值。方法:选择2型DN患者36例,正常对照组26例。收集临床血清样本,应用荧光定量PCR方法检测相应miRNA的含量,应用ROC曲线评价三者诊断DN的敏感度。结果:①糖尿病患者血清中miR-21和miR-192的水平均较对照组显著升高(P均<0.05),而miR-200c较对照组显著下降(P<0.05)。②miR-21、miR-192和miR-200c ROC曲线下的面积分别是0.822、0.736和0.751。结论:糖尿病患者血清中,miR-21,miR-192和miR-200c是早期诊断DN的敏感指标,有望用于临床监测。Objective：To detect the serum level of miR-21,miR-192 and miR-200c in patients with diabetic nephropathy.Methods： Thirty-six patients with type 2 diabetes nephropathy and 26 healthy subjects as controls were recruited.Total RNAs including miRNA was extracted from serum samples.The serum level of three miRNAs was detected by quantitative real-time PCR.Results： The serum level of miR-21and miR-192 were significantly up-regulated in patients with diabetic nephropathy,while miR-200c was down-regulated.The area under ROC curve of miR-21,miR-192 and miR-200c were 0.822,0.736 and 0.751 respectively.Conclusion： The three serum miRNAs are potential diagnostic serum markers in diabetic nephropathy patients are sensitive parameters in early diabetic nephropathy,and thus can be used in clinical monitoring.

关 键 词：微小RNA 糖尿病肾病 血清 检测 

分 类 号：R587.2[医药卫生—内分泌]