几种泥炭藓属植物苹果酸脱氢酶同工酶研究  

Study on Malate Dehydrogenase Isozyme of Several Sphagnum

作　　者：张春[1] 张以忠[1] 

机构地区：[1]毕节学院地理与生命科学学院,贵州毕节551700

出　　处：《毕节学院学报（综合版）》2012年第8期87-90,共4页Journal of Bijie University

摘　　要：采用聚丙烯酰胺凝胶电泳对泥炭藓属植物5个种10个居群的苹果酸脱氢酶同工酶进行分析,结果表明:苹果酸脱氢酶同工酶带共6条,MDH-5和MDH-6在5个种中均可见,其酶带明显,染色较深,说明酶活性较强,可以判断MDH-5和MDH-6为共有特征带。泥炭藓有酶带2条,卵叶泥炭藓有酶带5条,加萨泥炭藓有酶带6条,多纹泥炭藓有酶带4条,尖叶泥炭藓有酶带4条,通过SPSS系统聚类表明:泥炭藓属5泥炭藓植物种间存在遗传差异。The malate dehydrogenase isozymes of ten populations belonging to five species of Sphag- num were studied by means of polyacrylamide gel eletrophoresis （PAGE） . The results showed that there were six different hands of malate dehydrogenase isozyme. Among them, MDH-5 and MDH-6 were common and characteristic bands. Sphagnum palustre L. had two bands, Sphagnum ovatum Hamp. had five bands, Sphagnum khasiaum Mitt. had six bands , Sphagnum multifibrosum Li at Zang. and Sphagnum nemoreum Scop. had four bands. Spss systems clustering showed that there were genetic differences among five different species of Sphagnum.

关 键 词：泥炭藓 苹果酸脱氢酶同工酶 酶带 

分 类 号：Q946[生物学—植物学]