高效液相色谱法测定支扩养阴颗粒中蒙花苷的含量  

Determination of buddleoside in Zhikuoyangyin granules by HPLC

作　　者：吴文飞[1] 张利斌[1] 李玉平[1] 

机构地区：[1]同济大学附属上海市肺科医院药剂科,上海200433

出　　处：《药学实践杂志》2012年第4期307-309,共3页Journal of Pharmaceutical Practice

摘　　要：目的建立HPLC测定支扩养阴颗粒中蒙花苷的含量方法。方法色谱柱为DIKMA Platisil ODS柱(250 mm×4.6 mm,5μm),流动相为甲醇-水-醋酸(55∶44∶2),流速1.0 ml/min,进样量20μl,检测波长326 nm。结果蒙花苷在2.95～98.3μg/ml内线性良好,r=0.999 9(n=5),平均回收率100.2%,RSD为0.96%(n=15)。结论该法操作简便、灵敏、可靠,可用于支扩养阴颗粒中蒙花苷的含量测定。Objective To establish a high performance liquid chromatography method for the determination of buddleoside in Zhikuoyangyin granules. Methods The HPLC method was performed on a DIKMA Platisil ODS column（250 mm× 4.6 mm,5 μm） with the mobile phase consisting of methanol-water-acetic acid（55 ： 44 ： 2）. The flow rate was 1.0 ml/min. The sample injection volume was 20 μl. The detective wavelength was 326 nm. Results The calibration curve of buddleoside showed good linear response ranged from 2.95 to 98.3 μg/ml with r = 0. 999 9 （ n = 5）. The average recovery of spiked samples for buddleoside was 100.2% while the relative standard deviation for repetitions was 0.96% （ n = 15）. Conclusion The method was simple, sensitive, reliable and repeatable, and could be used for the quantitative determination of buddleoside in Zhikuoyangyin granules.

关 键 词：蒙花苷 小蓟 支扩养阴颗粒 含量测定 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]