敌百虫中毒对贵州白香猪氧化应激及自由基损伤的影响  

Effect of Dipterex on Oxidative Stress and Free Radical Damage of Guizhou Baixiang Pig

作　　者：张勇[1,2] 许厚强[1,2] 左康阳 杨春怒 徐彩龙[1,2] 李金杰[1,2] 

机构地区：[1]贵州大学动物科学学院,贵州贵阳550025 [2]高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州贵阳550025 [3]开阳县农业局,贵州贵阳550300

出　　处：《贵州农业科学》2012年第9期161-163,共3页Guizhou Agricultural Sciences

基　　金：贵州省自然科学基金"分子印迹技术应用于猪肉中阿维菌素类药物残留的检测研究"[黔科合J字(2008)2128];贵州省科技计划课题"贵州香猪研究平台建设"[黔科合NY(2007)3007];贵州大学青年科技基金项目"肉品中农药等有毒有害物质的快速检测及其遗传毒性研究"[贵大自青基合字(2007)074]

摘　　要：为探讨急性敌百虫中毒对贵州白香猪氧化应激和自由基损伤的影响,为贵州白香猪有机磷农药中毒诊断和治疗提供参考数据,采用兽用敌百虫对贵州白香猪进行经口急性毒性试验,观察试验猪临床中毒症状并用分光光度法检测其肝、脾、肺、肾等组织的乙酰胆碱酯酶(AchE)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、胆碱酯酶(CHE)活力及VC、VE水平。结果表明:中毒贵州白香猪体内存在严重氧化应激及自由基损伤;与对照相比,染毒处理试验猪的肝、脾、肺、肾中的AchE、CHE活力及VC、VE含量均呈下降趋势,SOD和POD活力则有所提高,说明,有机磷中毒导致乙酰胆碱酯酶活力受抑制,体内自由基反应加剧。Dipterex was used in acute toxicity tests by mouth in Guizhou Baixiang Pigs to study the acute poisoning mechanism of dipterex and relationship among acute dipterex poisoning, oxidative stress and free radical damage. The clinical poisoning symptoms of trial pigs were observed and the activities of acetylcholinesterase（AchE）, peroxydase（POD）, superoxide dismutase （SOD） and cholinesterase（CHE） as well as level of vitamin C and vitamin E in their liver, spleen, lung and kidney tissues were determined by spectrophotometry. The results showed that serious oxidative stress and free radical damage occured in Guizhou Baixiang Pigs when organophosphate poisoning. Compared with the control group, the AchE, CHE activity and Vc, VE content in liver, spleen, lung and kidney tissues of trial pigs all showed downtrend. However, their SOD and POD activity showed increasing trend, indicating that activity of Ache was inhibited and free radical reaction aggravation after organophosphate poisoning.

关 键 词：敌百虫 贵州白香猪 氧化应激 自由基损伤 

分 类 号：S859.82[农业科学—临床兽医学]