高效液相色谱-蒸发光散射检测器法测定甘桔冰梅片中桔梗皂苷D含量  

Determination of Kikyosaponi D in Ganjie Bingmei Tablets by HPLC-ELSD

作　　者：彭善贵[1] 文永盛[1] 

机构地区：[1]四川省成都市食品药品检测中心,四川成都610045

出　　处：《中国药业》2012年第19期28-29,共2页China Pharmaceuticals

摘　　要：目的建立测定甘桔冰梅片中桔梗皂苷D含量的高效液相色谱-蒸发光散射检测器法。方法色谱柱为Diamonsil C18柱(200 mm×4.6 mm,5μm),流动相为乙腈-水(26∶74),流速为1.0 mL/min;Waters 2424型蒸发光散射检测器,漂移管温度为85℃,气体流速为2.9 SLPM/min。结果桔梗皂苷D进样量在0.952～11.900μg范围内与峰面积线性关系良好(r=0.999 2),平均回收率为98.82%,RSD=1.74%(n=6)。结论所建立的方法测定甘桔冰梅片中桔梗皂苷D的含量准确、可靠、操作简便。Objective To determine the kikyosaponin D content in Ganjie Bingmei Tablets by HPLC evaporative light scattering detector （ELSD） method. Methods The analytical column was Diamonsil Cls column （200 mm x 4.6 mm, 5 μm）, with acetonitrile water（26 ： 74） as the mobile phase at the flow rate of 1.0 mL/min. The Waters 2424-ELSD was adopted for detection. The drift tube temperature was 85 ℃ and the gas flow rate was 2.9 SLPM/min. Results The sample size of kikyosaponin D in range of 0.952-11. 900 μg showed good linear relationship （ r = 0. 999 2）. The average recovery rate was 98.82%, RSD = 1.74% （ n = 6）. Conclusion This method for determining the kikyosaponin content is accurate, reliable and easy to operate.

关 键 词：甘桔冰梅片 高效液相色谱-蒸发光散射检测器法 桔梗皂苷D 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]