糖尿病大鼠骨骼肌组织丝氨酸-苏氨酸蛋白激酶B的表达及其与炎性细胞因子的关系  

Expression of serine/threonine protein kinase B and relationship with inflammation cell factors in skeletalmuscle of diabetic rats

作　　者：王君[1] 王志辉[1] 张译文[1] 

机构地区：[1]山东省青岛市第八人民医院内分泌科,266100

出　　处：《中国医药》2012年第10期1239-1240,共2页China Medicine

基　　金：山东省青岛市科技发展指导计划项目（KZJ-29）

摘　　要：目的探讨丝氨酸-苏氨酸蛋白激酶B（PKB）在糖尿病大鼠骨骼肌中的表达及其与炎性细胞因子肿瘤坏死因子仪（TNF-α）、白细胞介素6（IL-6）的关系。方法将清洁液Wistar大鼠完全随机分为实验组与对照组，各8只。实验组给予高脂、高糖饮食8周后腹腔内注射小剂量链脲佐菌素（30mg／kg），对照组喂养标准大鼠饲料。第13周末处死大鼠，取各组大鼠股四头肌用免疫组织化学方法检测PKB、TNF-α及IL-6的蛋白表达，并分析PKB表达与TNFα、IL-6表达的相关性。结果TNF-α在对照组骨骼肌上表达很少，几乎为0；实验组阳性细胞率为（3．8±1．4）％，2组比较差异有统计学意义（P〈0．05）。IL-6在对照组大鼠骨骼肌上表达率为（2．0±0．8）％；实验组阳性细胞率（5．8±0．7）％，与对照组比较差异有统计学意义（P〈0．05）。PKB在对照组大鼠骨骼肌上阳性率为（9．0±0．9）％；实验组PKB表达减少，阳性细胞率为（2．8±0．8）％，与对照组比较表达明显减少（P〈0．05）。TNF-α、IL-6表达与PKB的表达呈负相关（r=-0．776，r=-0．698，P〈0．05）。结论PKB信号传导系统可以抑制糖尿病大鼠骨骼肌中TNF-α和IL-6的释放。Objective To evaluate the changes of serine/threonine protein kinase B （ PKB）, tumor necrosis factor-α（TNF-α） and interleukin 6 （IL-6） expression in quadrieeps femoris muscle of diabetic rats, and to explore the relationship among them. Methods Adult Wistar rats were divided into experimental group （ n = 8 ） and control group （ n = 8 ）. Diabetic rats in experimental group were fed by high lipoid and glucose diet. After 8 weeks, they were made by a single injection of streptozotocin （STZ） at a dose of 30 mg/kg. After 13 weeks, they were killed for detecting PKB, TNF-α and IL-6 by quantitative immunohistochemistry assay. Correlation analysis was used to evaluate the relationship among them. Results The expression of TNF -α in the control group in skeletal muscle was almost 0 ; the positive cell rates in the experimental group was （ 3.8 ± 1.4 ） % and there was a significant difference between two groups （ P 〈 0. 05 ）. The expression of IL-6 in normal rat skeletal muscle was （ 2. 0 ± 0. 8 ） % ; the positive cell rate in the experimental group was （5.8 -± 0. 7 ） % , which showed a significant difference compared with the control group （ P 〈 0. 05 ）. The positive rate of PKB in skeletal muscle of control rats was （9. 0 ± 0. 9）%; the positive cell rate in the experimental group was （2. 8 ± 0. 8 ）%, which significantly decreased compared with the control group （P 〈 0. 05 ）. There was a significant correlation among PKB and TNF-α, IL-6 （ r = - 0. 776, r = - 0. 698, P 〈 0. 05 ）. Conclusion PKB pathway can inhibit the expression of the TNF-α and IL-6 in quadriceps femoris muscle of diabetic rats.

关 键 词：糖尿病 丝氨酸-苏氨酸蛋白激酶B 肿瘤坏死因子-Α 白细胞介素-6 

分 类 号：R587.1[医药卫生—内分泌]