软肝化坚颗粒对肝纤维化模型C57小鼠肝组织α-SMA、TGFβ1表达和I型胶原水平的影响  

Effects of Ruangan Huajian granule on hepatic expression of α-SMA,TGFβ1,and COL-Ⅰ in C57 mice with experimental hepatic fibrosis

作　　者：叶立红[1] 王翀奎[2] 杨莉[1] 高会霞[1] 赵召霞[1] 侯军良[1] 戴二黑[1] 

机构地区：[1]石家庄市第五医院检验科,石家庄050021 [2]河北医科大学西山校区,石家庄050200

出　　处：《临床肝胆病杂志》2012年第9期685-688,共4页Journal of Clinical Hepatology

基　　金：河北省中医药管理局科学技术研究计划基金项目(2008055)

摘　　要：目的观察软肝化坚颗粒对肝纤维化模型C57小鼠肝组织中α-平滑肌肌动蛋白(α-SMA)、转化生长因子(TGF)β1表达和I型胶原水平的影响。方法 40只C57小鼠随机分为4组,分别为正常对照组、模型对照组、软肝化坚颗粒低剂量组及高剂量组,每组10只。采用四氯化碳(CCl4)腹腔注射制造小鼠肝纤维化模型,通过免疫组织化学染色方法检测肝组织中α-SMA、TGFβ1和I型胶原的表达,同时进行肝组织纤维化评分(S)。结果各模型组肝组织α-SMA、TGFβ1及Ⅰ型胶原表达和纤维化评分与正常对照组比较,差异均具有统计学意义(P<0.05);软肝化坚颗粒低剂量组、高剂量组肝组织α-SMA和TGFβ1及Ⅰ型胶原评分和纤维化评分较模型对照组均明显降低,差异具有统计学意义(P<0.05);软肝化坚颗粒低剂量组和高剂量组α-SMA、TGFβ1及Ⅰ型胶原评分及肝纤维化评分之间差异无统计学意义(P>0.05)。结论软肝化坚颗粒可能通过抑制C57小鼠肝组织TGFβ1的产生,进而抑制肝星状细胞(HSC)的活化,减少细胞外基质(ECM)的合成,从而降低I型胶原增生,达到预防和治疗肝纤维化的作用。Objective To observe the effect of Ruangan Huajian granule on hepatic expression of alpha - smooth muscle actirl （ α - SMA）, tumor growth factor - beta 1 （TGFβ1）, and collagen Ⅰ （ COL - I） using a C57 mouse model of experimental hepatic fibrosis. Methods Forty C57 mice were randomly divided into four groups （n = 10 each） ： uninduced normal control group, model control group, model treatment group with low - dose （0.1 g/day for six weeks） Ruangan Huajian granule, model treatment group with high - dose （0.2 g/day for six weeks） Ruan- gan Huajian granule. Hepatic fibrosis was induced by intraperitoneal injection of 10% carbon tetrachloride. The expression of α - SMA, TGFβ1, and COL -Ⅰ in liver tissue of mice was observed by immunohistochemistry. The liver tissues were also scored for fibrosis stage by eval- uating morphological changes detected upon hematoxylin - eosin and Masson trichrome staining. Results There were significantly higher levels of α - SMA, TGFβ1, and COL - Ⅰ expression and scores of fibrosis stage in the hepatic fibrosis mice than in the normal control mice （ P 〈 0. 05 ） and in the model control group than in either the low - dose or high - dose model treatment groups （ P 〈 0.05 ）. There was no significant difference between the low - dose model treatment group and the high - dose model treatment group （ P 〉 0.05 ）. Conclusion Ruangan Hua- jian granule may alleviate the degree of fibrosis in C57 mice with experimental hepatic fibrosis by reducing hepatic expression of α - SMA and TGFβ1. Furthermore, Ruangan Huajian granule may depress hyperplasia by reducing COL- Ⅰ expression.

关 键 词：软肝化坚颗粒 肝硬化 α-平滑肌肌动蛋白 转化生长因子β Ⅰ型胶原 

分 类 号：R285.5[医药卫生—中药学]