肺结核患者外周血NK细胞数量和胞内杀伤分子的变化  

Changes of the number of NK cells and intracellular killing molecules in peripheral blood of patients with pulmonary tuberculosis

作　　者：邱晓静[1] 王兆华[2] 王健[3] 孙建忠 姚春艳[1] 李柏青[1] 

机构地区：[1]蚌埠医学院免疫学教研室,安徽蚌埠233030 [2]安徽省蚌埠市传染病医院呼吸科,233000 [3]安徽理工大学医学院病原生物学教研室,安徽淮南232001 [4]江苏省常州市武进人民医院检验科,213002

出　　处：《蚌埠医学院学报》2012年第10期1141-1144,共4页Journal of Bengbu Medical College

基　　金：国家科技重大专项基金课题资助项目(2008ZX10003-011)

摘　　要：目的:检测初治和复治结核病患者外周血NK细胞数量和胞内杀伤分子的变化。方法:取健康人(112名)和结核病患者145例(初治105例,复治40例)抗凝外周全血标本,加荧光标记抗CD3、CD56、CD8、颗粒酶和穿孔素抗体进行标记,进行流式细胞术检测。结果:初治肺结核患者外周血NK细胞的比例(16.95±8.27)%显著低于健康组(19.99±9.28)%(P<0.01)。健康组外周血NK细胞绝对值计数为(44.32±26.69)×104/ml,结核初治组为(33.31±21.44)×104/ml,复治组为(36.8±24.97)×104/ml,结核初治组明显低于健康组(P<0.01)。结核病患者NK细胞中表达颗粒溶解素(85.78±11.63)%,穿孔素(82.88±17.05)%,颗粒酶(79.07±15.64)%,健康组(87.31±10.34)%、(82.85±13.03)%、(88.04±7.27)%,2组颗粒溶解素和穿孔素差异均无统计学意义(P>0.05),而颗粒酶差异有统计学意义(P<0.01)。结论:初治组结核病患者外周血NK细胞数量明显降低,但NK细胞中颗粒溶解素、穿孔素的表达和健康组差异均无统计学意义。Objective： To detect the changes of the number of NK cells and intraceUular killing molecules in peripheral blood ofpatients with pulmonary tuberculosis. Methods：Percentages of NK cells in peripheral whole blood samples from 145 pulmonary TB patients（ 105 first-treated pulmonary TB and 40 retreated pulmonary TB） and 112 healthy controls were collected, labelled with antiCD3, CD56, CD8, granulysin, perforin and granzyme antibody, and analyzed by flow cytometry. Results： The percentage of NK cells in first-treated pulmonary TB patients was （ 16.95±8.27） %, which was significantly lower than that in healthy controls （ 19.99 ± 9.28 ） % （P 〈 0. 01 ）. Absolute value of NK cells in first -treated pulmonary TB patients was （33.31 ±21.44） × 10^4/ml, which was significantly lower than （hat in healthy controls （44.32 ± 26.69 ） × 10^4/ml） （ P 〈 0.05 ）. Absolute value of NK cells in retreated pulmonary TB patients was （36.8 ± 24.97 ）× 10^4/ml. Granulysin [ （ 85.78 ± 11.63 ） % vs （ 87.31 ± 10.34） % ], perforin [ （ 82.88± 17.05 ）% vs （82.85 ±13.03 ）% ] in NK cells had no difference bewteen pulmonary rib patients and healthy controls（P 〉 0.05 ）, but the difference of granzyme [ （79.07 ± 15.64 ） % vs （ 88.04 ± 7.27 ） % ] was significant （ P 〈 0.01 ）. Conclusions： The number of NK cells in peripheral whole blood of first-treated pulmonary TB patients NK was significantly decreased. However, the expressions of granulysin and pefforin in NK cells had no difference between pulmonary TB patients and healthy controls.

关 键 词：肺结核 自然杀伤细胞 颗粒溶解素 颗粒酶 穿孔素 

分 类 号：R521[医药卫生—内科学]