呼吸道给药诱导产生干扰素的实验研究  

Study on interferon induced by respiratory tract medication

作　　者：王洪波[1] 樊凤艳[1] 刘娟[1] 祁术元[1] 

机构地区：[1]空军总医院输血科,北京100142

出　　处：《海南医学》2012年第19期16-18,共3页Hainan Medical Journal

摘　　要：目的探讨干扰素(IFNs)诱导剂从鼻腔给药,能否诱导呼吸道局部黏膜产生足量干扰素,预防呼吸道病毒感染性疾病。方法选用干扰素诱导剂聚肌胞、新城疫疫苗、阿比朵尔和刺五加多糖溶液,连续15d从鼻腔滴入,然后检测实验动物血液和鼻腔分泌物中的干扰素含量。结果⑴各实验组动物血清中检测的干扰素含量比空白对照组明显低;鼻腔分泌物中检测的干扰素含量则比空白对照组明显高。说明通过鼻腔给予干扰素诱导剂,对血液中干扰素的产生无促进作用,对呼吸道黏膜干扰素的产生有诱导和促进作用。⑵与空白对照组比较,除刺五加多糖高剂量组差异无统计学意义(P>0.05)、刺五加多糖低剂量组差异有统计学意义(P<0.05)外,其他各实验组差异均有显著统计学意义(P<0.01)。⑶实验动物对鼻腔滴入上述制剂,有较好的适应性。结论聚肌胞、新城疫疫苗、阿比朵尔、刺五加多糖可以做为通过呼吸道给药的高效干扰素诱导剂,用于探讨预防和治疗人类呼吸道病毒感染性疾病。Objective To explore whether the interferon （IFNs） inducer through nasal administration can in- duce local respiratory mucosa to produce sufficient amount of interferon, and to prevent respiratory tract virus infec- tions. Methods Four interferon inducers, Poly I：C, Newcastle disease virus vaccine, Arbidol, Polysaceharides of Ac- anthopanax sentieobas （PAS） solution, were applied through nasal administration for 15 days, and then the levels of in- terferon in animal blood and nasal secretions were detected. Results （1） The detected interferon levels in the serum were significantly lower in the experimental groups than the control group, and that in the nasal secretions were signifi- cantly higher in the experimental groups than the control group, which indicated that interferon inducers trough nasal administration can not promote the production of interferon in the blood, but can induce and promote respiratory mu- cosa to produce interferon. （2） Compared with the control group, the detected interferon levels of Polysaccharides group with low dose （P〈0.05） and other experimental groups （P〈0.01） were significantly promoted （P〈0.05） except for Polysaccharides group with high dose （/9〉0.05）. （3） Experimental animals treated with above mentioned interferon inducers through nasal administration had good adaptability. Conclusion The four interferon inducers, Poly I：C, Newcastle disease virus vaccine, Arbidol, PAS solution through nasal administration can prevent and treat infections of respiratory tract virus.

关 键 词：干扰素 诱导剂 预防 呼吸道 病毒感染 

分 类 号：R183.3[医药卫生—流行病学]