CHO细胞初始接种密度对细胞毒性试验结果的影响  

Effect of CHO Cell Initial Seeding Density on the Results of Cytotoxicity Test

作　　者：管莹[1] 夭建华[1] 高茜[1] 曾婉俐 黄海涛[1] 李雪梅[1] 缪明明[1] 

机构地区：[1]云南烟草科学研究院,云南昆明650106

出　　处：《化学与生物工程》2012年第9期43-46,共4页Chemistry & Bioengineering

基　　金：中国烟草总公司资助项目(110200902060);云南省烟草化学创新团队建设项目(2009CI014);云南省应用基础研究项目(2009ZC190M);云南省中烟工业公司资助项目(2011JC02)

摘　　要：考察了CHO细胞初始接种密度对体外细胞毒性试验结果的影响。通过细胞倍增试验检测不同初始接种密度下CHO细胞倍增时间的差异,并分别采用中性红比色法及MTT比色法检测CHO细胞初始接种密度对细胞毒性试验结果的影响。结果表明,CHO细胞初始接种密度对细胞毒性试验的结果存在一定影响:以2×104～6×104个·mL-1为细胞初始接种密度,CHO细胞在48h内生长速度较为稳定;以4×104～6×104个·mL-1为细胞初始接种密度,中性红比色法毒性试验结果较为稳定;以2×104～4×104个·mL-1为细胞初始接种密度,MTT比色法细胞毒性试验结果较为稳定。在不同的细胞毒性试验中选择适合的细胞初始接种密度有助于提高细胞毒性试验结果的可重复性。The effect of CHO cell initial seeding density on the results of cytotoxicity test was investigated. The doubling time of CHO cells with different initial seeding densities were determined. Neutral red assay and MTT assay were used to test the eytotoxicity of different density cells. Results showed, with 2 ×104 -6 × 10^4 CFU · mL^-1 as the initial seeding density,the growth rate of CHO cell was relatively stable within 48 h,with 4 × 10^4-6 × 10^4 CFU , mL^-1 as the initial seeding density,neutral red toxicity test results was more reliable, with 2 × 10^ 4-4 ×10^4 CFU · mL^-1 as the initial seeding density,MTT toxicity test results was more reliable. The proper cell initial seeding density was beneficial to improving the reliability and repeatability of cytotoxicity test results.

关 键 词：细胞初始接种密度 中性红比色法 MTT比色法 细胞毒性 

分 类 号：R994.3[医药卫生—毒理学]