野生石蒜中石蒜碱和加兰他敏的分离研究  

Study on Separation of Lycorine and Galanthamine from Wild Lycoris

作　　者：熊海蓉[1] 李霞[2] 文祝友[3] 赵明明[2] 熊远福[2] 刘英[2] 

机构地区：[1]湖南农业大学分析测试中心,湖南长沙410128 [2]湖南农业大学理学院,湖南长沙410128 [3]湖南农业大学动物科技学院,湖南长沙410128

出　　处：《化学与生物工程》2012年第9期51-53,共3页Chemistry & Bioengineering

基　　金：湖南省科技厅计划项目(2011SK3082)

摘　　要：采用大孔树脂法纯化从野生石蒜中提取的石蒜总碱,用高速逆流色谱法进行分离,用高效液相色谱法进行检测。结果表明,高速逆流色谱法分离石蒜碱和加兰他敏的最佳溶剂体系为:正己烷∶乙酸乙酯∶甲醇∶水=3∶7∶3.5∶6.5(体积比),分离后石蒜碱的纯度为95.36%,加兰他敏的纯度为91.67%。高速逆流色谱法可以同时将石蒜总碱中石蒜碱和加兰他敏分离,分离效果良好。Macroporous resin was applied to purify alkaloids from Lycoris. The alkaloids was separated using high-speed counter-current chromatography （HSCCC） and was detected using high performance liquid chromatography. The results showed that the optimal solvent system for HSCCC to separate lycorine and galanthamine from Lycoris was hexane-ethyl acetate-methanol-water（3 ： 7 ： 3.5 ： 6. 5, volume ratio）. The purity of lycorine reached 95.36 % and the purity of galanthamine reached 91.67 % after separation. The high-speed counter-current chromatography was proved to be effective to separate lycorine and galanthamine simultaneously.

关 键 词：石蒜 石蒜碱 加兰他敏 分离 高速逆流色谱 

分 类 号：O629.3[理学—有机化学]