结核分枝杆菌噬菌体快速检测方法的临床应用评价  

Clinical application evaluation of the FAST plaque TB assay for rapid detection of Mycobacterium tuberculosis

作　　者：卿克勤[1] 韩敏[2] 乐军[2] 

机构地区：[1]成都市第一人民医院,成都610016 [2]同济大学附属上海市肺科医院,上海200433

出　　处：《检验医学》2012年第9期760-763,共4页Laboratory Medicine

摘　　要：目的评价噬菌体生物扩增法直接检测痰样本中结核分枝杆菌的临床效果。方法应用噬菌体生物扩增法、金胺O染色涂片镜检和快速培养法对530例临床痰样本进行检测。结果以快速培养法培养结果为参考标准进行评价。结果 530例痰样本中有270例快速培养法阳性,其中噬菌体生物扩增法有220例阳性,而260例快速培养法阴性的样本中有20例噬菌体生物扩增法阳性;噬菌体生物扩增法的敏感性、特异性、阳性预测值和阴性预测值分别为81.6%、92.3%、91.6%、82.8%。涂片联合噬菌体生物扩增法的检测敏感性、特异性、阳性预测值、阴性预测值分别为88.9%、80.8%、82.8%和87.5%。结论噬菌体生物扩增法具有较高的敏感性和特异性,以及快速、安全等优点,特别是对于痰涂片阴性的结核病患者具有较高的临床应用价值。Objective To evaluate the efficacy of FAST plaque TB assay for the direct detection of Mycobacterium tuberculosis in sputum specimens. Methods A total of 530 sputum specimens were detected by FAST plaque TB assay, auramine O stain smear microscopy and a rapid culture assay. Results of a rapid culture assay were as the reference standards for evaluation. Results Of the 530 sputum samples, there were 270 sputum samples positive by the rapid culture assay, including FAST plaque TB assay detected 220 positive. In the 260 negative sputum samples by the rapid culture assay, there were 20 positive by FAST plaque TB assay. The sensitivity, specificity, positive and negative predictive values of FAST plaque TB assay were 81.6% , 92.3% ,91.6% and 82.8%. The sensitivity, specificity, positive and negative predictive values of the combined determination of FAST plaque TB assay and smear microscopy were 88.9% , 80.8% , 82.8% and 87.5%. Conclusions FAST plaque TB assay has certain high sensitivity and specificity , as well as fast, safe and other advantages. The assay shows a high clinical significance especially for smear- negative tuberculosis patients.

关 键 词：结核分枝杆菌 噬菌体 快速培养法 

分 类 号：R378.91[医药卫生—病原生物学]