Kzp Regulates the Transcription of gata2 and pu.1 during Primitive Hematopoiesis in Zebrafish Embryos  

作　　者：Fang Liu Shaohua Yao Ting Zhang Chun Xiao Yanna Shang Jin Liu Xianming Mo 

机构地区：[1]Laboratory of Stem Cell Biology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China

出　　处：《Journal of Genetics and Genomics》2012年第9期463-471,共9页遗传学报（英文版）

基　　金：supported by the grants from the National Natural Science Foundation of China(Nos. 30971070,31071277 and 31000646);the Key Project of Chinese Ministry of Education(No.109136) to X.M.

摘　　要：Kaiso zinc finger-containing protein（Kzp）,a maternally-derived transcription factor,controls dorsoventral patterning during zebrafish gastrulation.Here,we uncovered a new function for Kzp in zebrafish embryonic primitive hematopoiesis.The depletion of kzp led to defects in primitive hematopoiesis including the development of the erythroid and myeloid lineages.On the other hand,overexpression of kzp caused the ectopic expression of gata1,gata2,and pu.1.Chromosome immunoprecipitation assays revealed that Kzp protein directly binds to gata1,gata2,and pu.1 promoters.Interestingly,the ectopic expression of gatal was able to rescue the erythroid,but not the myeloid lineage in kzp-depleted zebrafish embryos.gata1 expression controlled by Kzp was dependent on gatal during primitive erythropoiesis.Our results indicate that Kzp is a critical transcriptional factor for the expression of gata2 and pu.1 to modulate primitive hematopoiesis.Kaiso zinc finger-containing protein（Kzp）,a maternally-derived transcription factor,controls dorsoventral patterning during zebrafish gastrulation.Here,we uncovered a new function for Kzp in zebrafish embryonic primitive hematopoiesis.The depletion of kzp led to defects in primitive hematopoiesis including the development of the erythroid and myeloid lineages.On the other hand,overexpression of kzp caused the ectopic expression of gata1,gata2,and pu.1.Chromosome immunoprecipitation assays revealed that Kzp protein directly binds to gata1,gata2,and pu.1 promoters.Interestingly,the ectopic expression of gatal was able to rescue the erythroid,but not the myeloid lineage in kzp-depleted zebrafish embryos.gata1 expression controlled by Kzp was dependent on gatal during primitive erythropoiesis.Our results indicate that Kzp is a critical transcriptional factor for the expression of gata2 and pu.1 to modulate primitive hematopoiesis.

关 键 词：kzp Hematopoiesis Erythropoiesis Myelopoiesis Zebrafish 

分 类 号：Q753[生物学—分子生物学] S896.3[农业科学—特种经济动物饲养]