Comparative Studies on Detection of Antibodies against Infectious Bursal Disease Virus with Test Strips and Agar Gel Immunodiffusion Method  

试纸条法与琼脂扩散法检测IBDV抗体的比较(英文)

作　　者：张进良[1,2] 张文通[3] 胡思顺 毕丁仁[1] 王喜亮[1] 肖运才[1] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070 [2]商丘师范学院生命科学学院,河南商丘476000 [3]山东省农业科学院滨州分院山东省滨州畜牧兽医研究院,山东滨州256600

出　　处：《Agricultural Science & Technology》2012年第9期1826-1828,共3页农业科学与技术（英文版）

摘　　要：[Objective] This study aimed to compare the detection results of antibodies against infectious bursal disease virus with test strips and agar gel immunodiffusion method. [Method] Antibodies against infectious bursal disease virus in chicken serum were detected using test strips developed in our laboratory, and the results were comparad^with that using traditional agar diffusion method. [Result] The comparative study of the two methods showed that the sensitivity of test strips was eight times over agar gel immunodiffusion; test strips showed higher detection rate in the deter- mination test of 216 clinical samples, with high specificity, easy preservation, and simple and rapid operation, thereby being more suitable for the monitoring of clinical antibodies. [Conclusion] Test strips could replace the existing serological methods, having great promotion and application value in antibody monitoring.[目的]比较分析IBDV抗体的试纸条与琼脂糖扩散两种检测方法。[方法]用自行研制的试纸条法检测鸡血清中的鸡传染性法氏囊病毒抗体,并与传统的琼脂扩散法检测结果进行比较。[结果]试纸条较琼脂凝胶扩散试验敏感8倍,在216份临床样品测定试验中,检出率更高,同时其又具有特异性强、易保存、操作简单快速等优点,故试纸条更适合于临床抗体监测。[结论]该研究结果表明试纸条可替代现有的血清学方法,在抗体监测方面具有极大的推广应用价值。

关 键 词：Infectious bursal disease Agar gel immunodiffusion Test strips 

分 类 号：S858.31[农业科学—临床兽医学]