Screening on Synergist of Bacillus thuringiensis Wettable Powder  

苏云金杆菌可湿性粉剂增效因子的筛选(英文)

作　　者：葛东华[1] 张晓红[1] 南宫自艳[1] 宋萍[1] 王勤英[1] 曹克强[1] 

机构地区：[1]河北农业大学植物保护学院,河北保定071000

出　　处：《Agricultural Science & Technology》2012年第9期1915-1918,共4页农业科学与技术（英文版）

基　　金：Supported by Special Fund for Agro-scientific Research in the Public Interest(200903042-5);National Apple Industry Technology System Project of China(nycytx-08-04-01)~~

摘　　要：[Objective] This study aimed to screen the best synergistic material for Bt wettable powder and evaluate their synergistic effect. [Method] The synergism of six different kinds of additives for Bacillus thuringiensis wettable powder （Bt WP） on the 2^nd instar larvae of Plutella xylostella was tested by method of leaf dipping in labora- tory. [Result] The mixtures of Bt with 0.1% ZnCl2, 0.5% ZnCl2, 1.0% ZnCl2, 1.0% MgCI2, 0.5% boric acid, 1.0% boric acid, 0.5% citric acid or 1.0% citric acid all ex- hibited synergistic effect, in which the synergistic effect of mixture containing 0.5% boric acid was the highest, with 17.2 synergistic ratio; followed by the mixture containing 1.0% ZnCl2, with 15.6 synergistic ratio. Moreover, addition of 0.5% boric acid could shorten the median lethal time of Bt wettable powder by about 10 h. After the mixtures of Bt with 0.5% boracic acid or 1.0% ZnCl2 was stored for 15 d at room temperature, toxicities of the two mixtures did not change significantly. [Conclusion] Boracic acid as the synergist of Bt wettable powder could not only increase insecti- cidal effect of Bt, but also accelerate its insecticidal rate. So, boracic acid could improve the disadvantages of Bt wettable powder such as poor insecticidal effect and slow insecticidal speed in a certain degree.[目的]筛选对苏云金芽孢杆菌(Bacillusthuringiensis,简称Bt)可湿性粉剂具有最佳增效作用的物质,并对增效作用显著的混配组合进行了增效作用的评定。[方法]以小菜蛾为生物测定昆虫,采用浸叶法测定了6种添加物对苏云金杆菌(Bacillusthuringiensis,Bt)可湿性粉剂的毒杀增效作用。[结果]研究发现0.1%ZnCl2、0.5%ZnCl2、1.0%ZnCl2、1.0%MgCl2、0.5%硼酸、1.0%硼酸、0.5%柠檬酸和1.0%柠檬酸对Bt可湿性粉剂表现出增效作用,其中0.5%硼酸对Bt可湿性粉剂的增效作用最强,增效比为17.2,1.0%ZnCl2次之,增效比为15.6。同时,杀虫速度的测定结果显示,0.5%硼酸的加入使Bt可湿性粉剂的致死中时缩短了约10h,加快了Bt的杀虫速度。此外,当将1.0%ZnCl2和0.5%硼酸分别与Bt可湿性粉剂溶液室温下混合放置15d后,两种混合物的毒力均没有明显的变化。[结论]硼酸作为Bt可湿性粉剂的增效因子,不但有利于增强Bt制剂的杀虫效果而且还可以提高其杀虫速度,从而在一定程度上克服了Bt制剂杀虫效果差和杀虫速度慢等实际问题。

关 键 词：Bacillus thuringiensis Synergism Cotoxicity factor Plutella xylostella 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]