林麝毛发DNA的提取及系统发育分析  

Hair DNA Extraction and Phylogenic Analysis of Forest Musk Deer(Moschus berezovskii)

作　　者：冯慧[1] 冯成利[1] 刘晓农[1] 任轶[1] 

机构地区：[1]陕西省动物研究所,西安710032

出　　处：《西北农业学报》2012年第8期14-18,共5页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：陕西省重大科技专项(2010ZDKG-54);陕西省科学院科技专项(2009K-02);陕西省科学技术研究发展计划项目(2012KW-21);陕西省科学院青年人才培养项目(2012K-027;2009K-25)

摘　　要：针对林麝及麝属动物系统分类存在较大争议的问题,采用分子标记方法,分析林麝的系统发育。利用非损伤取样方法,从秦岭林麝的毛发样品中提取得到线粒体DNACytb基因的部分序列,并对其进行分析。结果表明,林麝、原麝、马麝、喜马拉雅麝、黑麝是5种独立的种,林麝与原麝的亲缘关系最近。秦岭林麝种群的遗传多样性较低,单倍型多样度(Hd)为0.558,核苷酸多样度(Pi)为0.015 42。可弥补现有形态分类研究的不足,得到更有说服力的分析结果。According to the confusion of system classification of Moschus berezovskii, the phylogenetic of musk deer has been analyzed by the method of molecular marker. Partial DNA sequence of cyto-chrome b gene in mitochondrial genome was successfully retrieved from hair of forest musk deer col-lected from Shaanxi. Phylogenetic analysis using the sequence and published data indicated that forest musk deer, siberian musk deer, alpine musk deer, himalayan musk deer and tawny musk deer are five independent species. The forest musk deer has a close phylogenetic relationship to siberian musk deer and its haplotype diversity （Hd） was 0. 558, nucleotide diversity （Pi） was 0. 015 42. It is suggested that the forest musk deer has low genetic diversity.

关 键 词：林麝 线粒体DNA 系统发育 遗传多样性 

分 类 号：S865.41[农业科学—野生动物驯养]