8-羟基喹啉与鲱鱼精DNA作用方式的光谱法研究  

Study on the interaction mode between 8-Hydroxyquiuoline and DNA by spectrometry

作　　者：杨欢[1] 王兴明[1] 

机构地区：[1]西南科技大学材料科学与工程学院化学系,四川绵阳621010

出　　处：《商丘师范学院学报》2012年第9期49-52,共4页Journal of Shangqiu Normal University

基　　金：国家自然科学基金资助项目(30973634)

摘　　要：在pH=7.40的Tris-HCl缓冲溶液中,采用荧光光谱法和粘度法研究了8-羟基喹啉(HQ)与鲱鱼精DNA的作用方式.用摩尔比法确定了HQ与DNA的结合比为12∶1.通过热力学研究得出,在27℃时HQ与DNA之间相互作用的Kφ27℃=3.844×105L/mol,热力学函数ΔrHφm=-5.08×104J/mol,ΔrGφm 300.15K=-3.2×104J/mol,ΔrSφm=-62.6 J/(mol.K).结果显示该反应为焓驱动.实验表明HQ与DNA的作用方式为部分嵌插与静电作用方式.The mechanism of interaction between HQ and DNA has been studies by fluorescence spectra and viscosi- ty measurements in solution of Tris - HC1 （ pH = 7.4）. From mole ration method, the mole ration between HQ and DNA is 12： 1. Through the study of the thermodynamic we known that binding constant of HQ interaction with DNA is K25℃ = 3. 844 × 105 L/mol, the corresponding thermodynamic parameters are △rHm ： - 5. 08 × 104 J/mol, △rGm300.15K =-3.2 × 104 J/mol, △rSm = -62.6 J/（mol · K）. The result suggested that enthalpy was the driven force in this reaction. And the interaction of HQ and DNA through by electrostatic interaction and partial intercalation method.

关 键 词：鲱鱼精DNA 8-羟基喹啉 溴化乙锭 荧光光谱 作用方式 

分 类 号：O657.31[理学—分析化学]