Tγδ17和Tγδ22细胞在健康成人外周血中的比例  

The proportions of Tγδ17 cells and Tγδ22 cells in healthy adult peripheral blood

作　　者：徐丹[1] 梁华[1] 丁艳[1] 邱晓静[1] 盛玲玲[1] 李柏青[1] 

机构地区：[1]蚌埠医学院免疫学教研室安徽省感染与免疫重点实验室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2012年第9期1009-1012,共4页Journal of Bengbu Medical College

基　　金：国家自然科学基金资助项目(81072426)

摘　　要：目的:观察健康成人外周血中αβT细胞和γδT细胞中辅助性T细胞17(TH17)样细胞和TH22样细胞的比例。方法:采集18名正常人外周血用荧光抗体染色后用流式细胞仪检测αβT细胞、γδT细胞中白细胞介素-22(IL-22)、IL-17和γ干扰素(IFN-γ)表达细胞的百分比。结果:将正常人外周新鲜全血中CD3+T细胞分成γδT细胞和αβT细胞两群。TH1样细胞在αβT细胞中比例为14.31%,明显低于在γδT细胞中的61.07%(P<0.01),而TH17样细胞在αβT细胞中的比例为1.16%,明显高于在γδT细胞中的0.27%(P<0.01),TH22样细胞在αβT细胞中与γδT细胞中的比例分别为1.06%和0.91%,差异无统计学意义(P>0.05)。结论:在γδT细胞中存在IL-17和IL-22均阳性的Tγδ17细胞和仅产生IL-22的Tγδ22细胞,但比αβT细胞中的TH17样细胞和TH22样细胞少。Objective：To observe the proportions of TH17-like cells and TH22-like cells in αβT cells and γδT cells in healthy adult peripheral blood.Methods：Eighteen healthy adult peripheral blood samples were stained with fluorescent antibody and the percentages of interleukin-22,interleukin-17 and IFN-γ-producing cells in αβT cells and γδT cells were detected by flow cytometry.Results：The CD3＋T cells in the whole blood cells were divided into two groups：αβT cells and γδT cells.The proportions of TH1-like cells,TH17-like cells and TH22-like cells in αβT cells were 14.31%,1.16% and 1.06%,respectively.While the proportions of TH1-like cells,TH17-like cells and TH22-like cells in γδT cells were 61.07%,0.27% and 0.91%,respectively.The proportion of TH1-like cells in αβT cells was lower than in γδT cells,and the proportion of TH17-like cells in αβT cells was higher than in γδT cells（P〈0.01）.There were no statistical differences about the proportion of TH22-like cells in αβT cells and in γδT cells（P〉0.05）.Conclusions：There exists IL-17 and IL-22 double-positive Tγδ17 cell subpopulation and IL-22 single-positive Tγδ22 cell subpopulation in γδT cells.But the proportions are lower than those in αβT cells.

关 键 词：外周血 ΓΔT细胞 ΑΒT细胞 

分 类 号：R331[医药卫生—人体生理学]