超临界二氧化碳萃取法与水蒸气蒸馏法提取缬草油的化学成分比较  

Comparative Study on Constituents of Valerian Oil from CO_2 Supercritical Fluid Extraction and Steam Distillation Extraction

作　　者：方颖[1] 丁菲[1] 邬兰[1] 廖鹏程[1] 张慧慧[1] 刘焱文[1] 

机构地区：[1]湖北中医药大学中药资源与中药复方教育部重点实验室,武汉430061

出　　处：《医药导报》2012年第9期1116-1121,共6页Herald of Medicine

基　　金：国家自然科学基金资助项目(30500053);湖北省教育厅科学技术研究计划(Q20101806)

摘　　要：目的对不同提取方法提取的缬草油化学成分进行比较研究。方法采用超临界二氧化碳(CO2)萃取法和水蒸气蒸馏法从缬草中提取缬草油,用气相色谱-质谱联用(GC-MS)法进行化学成分定性与相对含量的比较。结果共鉴定了118种成分,超临界CO2萃取法提取物共鉴定98种,水蒸气蒸馏法提取鉴定了67种,共有成分47种;超临界CO2萃取所得缬草油的收率约为水蒸气蒸馏收率的1.8倍。结论超临界CO2萃取法是缬草油较好的提取方法。Objective To comparatively study the chemical constituents of valerian oil which were extracted with different extraction methods.Methods Valerian oil was seperated from valerian by CO2 supercritical fluid extraction or steam distillation extraction.Qualitative study on components and comparison of relative contents were carried out by GC-MS.Results A total of 118 chemical constituents were identified,98 kinds were obtained and identified via a CO2 supercritical fluid extraction,67 kinds were through steam distillation extraction,among which 47 kinds of constituents were the same.The yield of valerian oil which was extracted by CO2 supercritical fluid extraction was 1.8 times of that by the steam distillation extraction.Conclusion The results show that CO2 supercritical fluid extraction is more suitable for seperating valerian oil.

关 键 词：缬草 气相色谱-质谱联用法 超临界二氧化碳萃取法 水蒸气蒸馏法 

分 类 号：R282.71[医药卫生—中药学] R284.2[医药卫生—中医学]