不同地域东亚钳蝎蝎毒的双向电泳鉴定  

Identification of Scorpion Venom in Buthus Martensii Karsch from Different Regions by Two-dimensional Gel Electrophoresis(2-DE)

作　　者：倪伊婷[1] 郭美华[1] 王君德[2] 刘赟[3] 辛毅[1] 吴大畅[1] 

机构地区：[1]大连医科大学生物技术系,116044 [2]大连医科大学国有资产管理处,116044 [3]天津医科大学2008级医学检验专业,300070

出　　处：《医药导报》2012年第9期1200-1202,共3页Herald of Medicine

摘　　要：目的利用蛋白质组学中双向电泳技术,定性鉴定、分析不同地域东亚钳蝎蝎毒活性的差别,探索不同产地蝎毒的蛋白质组成及功能差异。方法将不同产地的冷冻干燥蝎毒粉经溶解、除盐、浓缩后测定蝎毒蛋白质含量,进行定量的蝎毒鉴定。采用pH梯度等电聚焦和SDS-PAGE凝胶电泳技术分离蝎毒蛋白质。银染后通过凝胶成像系统获得双向电泳凝胶图谱,用PD Quest图像分析软件比较分析,确定差异的特征蛋白点,从而定性鉴定不同产地蝎毒。结果获得3个样品的蛋白质指纹图谱。分别检测出80,69和77个点,特征差异蛋白点依次为56,46和55个。结论不同产地的东亚钳蝎蝎毒通过双向电泳分离蛋白后,表现出明显不同的蛋白点分布。Objective To identify qualitatively and analyze the activity of scorpion venom in Buthus Martensii Karsch from different regions by two-dimensional gel electrophoresis（2-DE）,and investigate the protein composition and function differences of scorpion venom.Methods Quantitive identification and content determination of proteins in scorpion venom were performed following preconditions including dissolution,desalting and condensing of the freeze-drying scorpion venom powder.The proteins in scorpion venom were separated by pH gradient isoelectric focusing and SDS-PAGE gel electrophoresis.The two-dimensional electrophoresis gel map was captured via gel imaging system after silver staining.The special different proteins were determined and comparatively analyzed through PD Quest image analysis software,and thus the scorpion venom from different regions was qualitatively identified.Results Protein fingerprints were acquired from three samples.Total protein spots were 80,69,77,and the distinctive number of which were 56,46,55,successively.Conclusion Scorpion venom in Buthus Martensii Karsch from different regions separated with 2-DE show obviously diverse protein distributions.

关 键 词：蝎毒 东亚钳蝎 双向电泳 鉴定 

分 类 号：R282.74[医药卫生—中药学] R927[医药卫生—中医学]