高效液相色谱法测定熊果酸原料中有关物质含量  

Determination of Related Substances in Raw Materials of Ursolic Acid by HPLC

作　　者：相延英[1] 金晓东[1] 张燕[1] 王腾[2] 张梦洁[2] 张长弓[2] 

机构地区：[1]解放军第161医院药剂科,武汉430010 [2]华中科技大学同济医学院药学院,武汉430030

出　　处：《医药导报》2012年第9期1205-1207,共3页Herald of Medicine

摘　　要：目的建立高效液相色谱(HPLC)法测定熊果酸原料中的有关物质含量,为制定其质量标准提供依据。方法采用Agilent XDB-C18(250 mm×4.6 mm,5μm)色谱柱,流动相为水相[水∶三乙胺∶冰醋酸(10∶0.03∶0.06)]与甲醇系统,梯度洗脱,检测波长210 nm,柱温30℃,流速1.0 mL·min-1,进样量20μL。结果熊果酸中齐墩果酸含量均<0.5%;其他杂质均未检出。结论该方法可以作为熊果酸原料中的有关物质的检查方法,简便,快速,准确,专属性好。Objective To establish a method for determination of related substances of ursolic acid raw materials and provide the evidence for its quality control.Methods The chromatographic column was Agilent XDB-C18（250 mm×4.6 mm,5 μm）.The mobile phase consisted of methanol-water phase [water：triethylamine：glacial acetic acid（10：0.03：0.06）].The gradient elution was applied as a chromatographic separation,and the detection wavelength was set at 210 nm.Results The oleanolic acid contained in the ursolic acid was less than 0.5%,and other impurities were not detected.Conclusion This method is simple,rapid,accurate and has a good specificity that can be used in checking the related substances in raw materials of ursolic acid.

关 键 词：熊果酸 齐墩果酸 有关物质 色谱法 高效液相 

分 类 号：R979.1[医药卫生—药品] R927.2[医药卫生—药学]