HPLC梯度波长法同时测定甘肃省不同产地甘草中6种成分含量及主成分分析研究  

HPLC Gradient Wavelength Method for the Simultaneous Determination of 6 Ingredients in Glycyrrhizae Radix et Rhizoma Produced in Different Regions in Gansu and Principal Component Analysis

作　　者：尚晓娜[1] 宋平顺 杨锡 何禄仁 赵建邦[1] 丁永辉 

机构地区：[1]兰州大学药学院,甘肃兰州730000 [2]甘肃省食品药品检验所,甘肃省中药品质与安全评价工程技术研究中心,甘肃兰州730000 [3]甘肃省食品药品监督管理局,甘肃兰州730000

出　　处：《中国现代中药》2012年第9期27-31,共5页Modern Chinese Medicine

基　　金："十一五"国家科技支撑计划项目--中药标准物质研制和开发的技术平台(2009ZX09308-001);甘肃省科技重大专项计划项目--当归等八种道地药材的质量标准制订(1002KFDA048)

摘　　要：目的:建立HPLC同时测定甘草中甘草苷、甘草素、异甘草素、甘草查尔酮A、甘草酸和甘草次酸含量的方法,应用主成分分析识别甘草的质量。方法:以乙腈-0.1%的磷酸水溶液进行流动相梯度洗脱,梯度波长采集法,柱温为室温,流量0.6mL·min-1;用spss17.0软件对数据进行分析。结果:色谱峰分离效果良好,甘草苷、甘草素、甘草酸铵、异甘草素、甘草查尔酮A和甘草次酸在各自浓度范围内均具有良好的线性关系。主成分分析对不同产地的样品排序结果与传统评价基本相符合。结论:本实验建立的HPLC同时测定黄酮类、三萜类成分的含量方法准确、简便,可作为甘草质量控制的方法之一。Objective： A HPLC isoliquiritigenin, licorice chalcone A, quantitatively method was used to determinate glycyrrhizin, liquiritigenin, glycyrrhizin and glycyrrhetinic acid in Glycyrrhizae radix et rhizoma simultaneously and to identify the quality of licorice by aqueous solution of phosphoric acid was used as mobile gradient-wavelength. The flow rate was 0. 6 mL-min1 principal component analysis. Methods： Acetonitrile-0. 1% phase for gradient elution and using collection method with and data analysis was used by SPSS 17.0. Results： Six Ingredients in Glycyrrhizae radix et rhizoma were separated well. They had good linear relationships in their respective range of concentration. Evaluation results of samples from different areas using principal component analysis were almost consistent with that by traditional evaluation. Conclusion： The method was accurate and simple. It could be used for quality control of Glycyrrhizae radix et rhizoma

关 键 词：甘草 黄酮类 三萜类 HPLC 梯度波长采集 主成分分析 

分 类 号：R284.1[医药卫生—中药学]