中医痰证与载脂蛋白A5基因-1131T>C多态性的关系研究  

Study on the relationship between apolipoprotein A5 gene-1131T>C polymorphism and phlegm stagnation syndrome

作　　者：聂娅[1] 周小青[1] 郭春秀[1] 黎鹏程[1] 谢宇霞[1] 

机构地区：[1]湖南中医药大学中医学院,湖南长沙410208

出　　处：《现代中西医结合杂志》2012年第26期2855-2857,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：湖南省教育厅科研基金资助项目(09C726);湖南中医药大学中医诊断学;中医基础理论资助项目

摘　　要：目的探讨载脂蛋白A5(ApoA5)-1131T>C基因多态性与中医痰证的关系。方法运用聚合酶链反应-限制性片段长度多态性分析技术对湖南地区131例痰证患者及74例健康人的ApoA5-1131T>C基因型进行检测,同时检测所有研究对象的血脂、血压及体质量指数。结果 205例受检者中,ApoA5-1131T>C位点TT型76例(37.07%),TC型94例(45.85%),CC型35例(17.07%),T等位基因频率60.00%,C等位基因频率40.00%。CC型血清三酰甘油水平明显高于TT型(P<0.01)。病例-对照研究及多因素Logistic回归分析均支持ApoA5-1131C是痰证发生的独立危险因素。结论 ApoA5-1131C可能是痰证发生的独立危险因素。ApoA5-1131CC型可能与血清三酰甘油水平升高有关。Objective It is to explore the relationship between the polymorphisms of apolipoprotein A5 （APOA5） gene （ -1131T 〉 C ） and phlegm stagnation syndrome（PSS）. Methods Patients of PSS（ n = 131 ） and healthy controls（ n = 74） were grouped and compared in this study. Using polymerase chain reaction-restriction fragment length polymorphisms （PCR - RFLP） technique to analyze the APOAS（ -I131T 〉 C） genotypes. Serum lipids, blood pressure（BP） and body mass index（BMI） in the pa- tients and controls were also measured. Results The frequencies of TT, TC, CC genotypes for the APOA5 - 1131T 〉 C poly- morphism were 37.07% （ n = 76） , 45.85% （ n = 94） and 17.07% （ n = 35）. The frequency of T allele was 60.00% and the frequency of C allele was 40.00%. The serum triglyceride （TG）level of - 1131 CC genotype carries were significantly higher than -I131TT genotype carries （P 〈 0.01 ）. Patients-controls research and muhinomial-logistic analysis indicated that- 1131 C was a risk factor of the PSS. Conclusion The - 1131 C allele of APOA5 gene is possibly the independent risk factor of the SPS. The - 1131CC genotype is possibly associated with the higher level of serum triglyceride.

关 键 词：痰证 载脂蛋白A5 基因多态性 三酰甘油 

分 类 号：R255.38[医药卫生—中医内科学]