茜草双酯对缺氧/复氧心肌细胞的保护作用研究  

The protection research of Rubidate for the hypoxia /reoxygenation injury of the cardiomyocytes

作　　者：黄桥华[1] 刘心强[1] 邱倩[1] 

机构地区：[1]赣南医学院第一附属医院,341000

出　　处：《中国现代药物应用》2012年第19期13-14,共2页Chinese Journal of Modern Drug Application

摘　　要：目的探讨茜草双酯对缺氧复氧的乳鼠心肌细胞是否具有保护作用。方法原代培养乳鼠心肌细胞,建立缺氧复氧模型,分别测各组细胞乳酸脱氢酶(LDH)活性、丙二醛(MDA)含量及细胞的凋亡与坏死。结果与缺氧/复氧模型组比较发现,缺氧/复氧模型预先加茜草组较缺氧/复氧模型组LDH活性和MDA含量降低有统计学意义(P<0.05),细胞凋亡和坏死有所减少。结论茜草双酯对体外培养的缺氧/复氧心肌细胞具有保护和抗凋亡作用。Objective To investigate if Rubidate can protect myocardium cells which accompany with hypoxia/reoxygenation in neonatal rat myocardial. Methods Myocardium cells of neonatal rat were primary cul-tured, hypoxia and reoxygenation model were established, activity of lactate dehydrogenase （ LDH）, malondial-dehyde （ MDA）, and rates of cell apoptosis and necrosis were detected respectively. Results Compared with hypoxia / reoxygenation model group, we found that the activity of LDH and MDA in hypoxia / reoxygenation- madder group were reduced more than hypoxia / reoxygenation group, the difference was statistically significant （P 〈 0. 05 ）. The rates of cell apoptosis and necrosis were less in hypoxia / reoxygenation-madder group than hypoxia / reoxygenation group. Conclusion Rubidate can protect myocardium cells which accompany with hy-poxia/reoxygenation and inhibiting cell apoptosis in vitro.

关 键 词：茜草双酯 缺氧/复氧 心肌细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]