野生二粒小麦导入普通小麦抗白粉病基因的分子标记  

Molecular Mapping of a Powdery Mildew Resistance Gene Transferred from Wild Emmer into Common Wheat

作　　者：沈红霞[1] 辛明明[1] 梁荣奇[1] 解超杰[1] 杨作民[1] 刘志勇[1] 孙其信[1] 

机构地区：[1]中国农业大学农业生物技术国家重点实验室,农业部作物基因组学与遗传改良重点开放实验室,北京市作物遗传改良重点实验室,教育部作物杂种优势研究与利用重点实验室,中国农业大学农学与生物技术学院,北京100193

出　　处：《华北农学报》2012年第4期7-11,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家"973"项目(2009CB118300);国家"863"项目(2006AA10Z1C4)

摘　　要：普通小麦的抗病品种(系)7K65对当前流行的小麦白粉菌E09生理小种具有较好的抗性,遗传分析表明,其抗性由一个显性基因控制,命名为Ml7K65。利用SSR,EST-STS标记对该基因进行检测,共筛选到7个与之连锁的标记位点,它们在遗传图谱上的顺序为:Xcfa2086-XEST83-XEST89-Xksum193-Xmag633-Xcfd50-Ml7K65,各位点之间的遗传距离依次为5.4,2.8,0.4,2.4,0.4,2.0 cM。其中,Xcfd50为离Ml7K65距离最近的标记,图距为2.0 cM,最远标记Xcfa2086与Ml7K65图距为13.4 cM。进一步利用中国春缺四体材料对该基因进行染色体定位,结果表明,Ml7K65基因位于小麦染色体2A上。Wheat line 7K65 was resistant to Blumeria graminis f. sp. tritici Race E09. Genetic analysis showed that the resistance was controlled by a single dominant gene,named MlTK65. After screening SSR,EST-STS markers,we found 7 marker loci were linked to the powdery mildew resistance gene Ml7K65. The genetic map of gene Ml7k65 indicates the order of these marker loci is Xcfa2086-XEST83-XEST89-Xksum193-Xmag633-Xcfd50-Ml7K65. The genetic distances of each interval are 5.4,2.8,0.4,2.4,0.4,2.0 cM, respectively. Xcfd50 is the closest marker, the genetic distance is 2.0 cM,while Xcfa2086 is the most distant maker with the genetic distance of 13.4 cM. Using Chinese Spring nullisomic-tetrasomic lines, we physically located the markers linked to Ml7K65gene on wheat chromosome 2A.

关 键 词：普通小麦 抗白粉病基因 分子标记 SSR标记 EST标记 

分 类 号：S435[农业科学—农业昆虫与害虫防治]