牦牛VEGF-A基因的克隆及生物信息学分析  

Cloning and Bioinformatics Analysis of VEGF-A Gene in Yak

作　　者：吴晓云[1,2,3] 阎萍[2,3] 梁春年[2,3] 郭宪[2,3] 包鹏甲[2,3] 裴杰[2,3] 褚敏[2,3] 丁学智[2,3] 刘文博[2,3] 焦斐[2,3] 刘建[2,3] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,甘肃兰州730050 [3]甘肃省牦牛繁育工程重点实验室,甘肃兰州730050

出　　处：《华北农学报》2012年第4期48-53,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家肉牛牦牛产业技术体系(CARS-38);公益行业农业科技专项(201003061);中央公益性科研院所基本科研业务费(1610322009002);青年科学基金项目(31101702)

摘　　要：利用分子克隆技术获得了牦牛VEGF-A基因编码区序列,并采用生物信息学方法对该基因及其编码蛋白的基本理化性质、疏水性、信号肽、二级结构等方面进行了预测和分析。结果表明,牦牛VEGF-A基因包含一个573 bp的开放阅读框,编码190个氨基酸;其编码蛋白属于亲水性蛋白,具有明显的信号肽。二级结构主要以无规则卷曲和α螺旋为主。VEGF-A基因编码产物氨基酸邻接系统树表明,牦牛VEGF-A与黄牛、绵羊和成都麻羊等物种的VEGF-A氨基酸遗传距离较近,具有高度同源性。A coding region sequence of yak VEGF-A was cloned by molecular cloning technique in this study. Some characters of the VEGF-A gene and encoded protein sequences were predicted and analyzed by the methods of bioinformatics in the following aspects as the general physical and chemical properties, hydrophobicity, signal pep- tide and secondary structure. Results showed that the full-length of yak VEGF-A contains a complete ORF（573 bp） encoding 190 amino acid. The protein encoded by yak VEGF-A has obvious signal peptide,it is a hydrophilic pro- tein. The secondary structures are mainly composed of Helix and Coli. The VEGF-A and related proteins of Bos grunnien,Bos taurus,Ovis aries,Capra hircus are close in the phylogenetic tree,which showed highly homology.

关 键 词：牦牛 VEGF—A基因 克隆 生物信息学分析 

分 类 号：Q78[生物学—分子生物学]