小麦RIL群体第一部分同源群染色体遗传多样性与偏分离分析  

Segregation Distortion and Genetic Diversity in Homoeologous Group 1 of Wheat RIL Population

作　　者：安梅[1] 杨德龙[1] 栗孟飞[1] 张国宏[2] 李唯[1] 

机构地区：[1]甘肃省干旱生境作物学重点实验室、甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃省农业科学院旱地农业研究所,甘肃兰州730070

出　　处：《华北农学报》2012年第4期73-79,共7页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(30960195);教育部科学技术研究重点项目(211183);甘肃省农牧厅生物技术专项(GNW-2008-10);甘肃省科技支撑计划项目(1104NKCA095)

摘　　要：为了明确小麦第一同源群染色体的遗传多样性,用小麦重组近交系群体(RIL)(Q9086×陇鉴19)108个株系为材料,利用SSR标记对小麦第一同源群进行遗传多样性和偏分离分析。结果表明,97对SSR引物在RIL群体亲本间筛选具有多态性标记23对,多态性频率1B(30.30%)>1A(28.57%)>1D(10.34%)。在RIL群体中检出107个等位位点有多态性,每个引物可扩增出2～10个位点,平均4.65个。每个位点多态信息含量(PIC)在0.326～0.880之间,PIC值1B(0.709)>1A(0.534)>1D(0.498)。株系间遗传相似系数(GS)在0.40～0.96之间。通过聚类可将RIL群体分为九大类。亲本基因型在群体中的分离比例为1∶1.13。通过χ2检测,有15个SSR标记表现为偏分离(P<0.05),偏分离频率为65.2%,其中,有7个标记偏向父本陇鉴19,8个标记偏向母本Q9086;6个标记偏分离在1A上,7个偏分离在1B上,2个分布在2D上。To know better the genetic diversity in homoeologous group 1 of wheat, a recombinant inbred line （RIL） population derived from a cross between two winter wheat cultivars, Longjian 19 and Q9086, was used to study the segregation distortion and the genetic diversity in homoeologous group 1 of wheat by SSR marker. The re- suits showed that twenty three pairs of SSR markers selected in 97 pairs of SSR primers were polymorphic between two parents. The polymorphic frequency in different chromosomes of homoeologous group 1 presented 1B （30.30%） 〉 1A （28.57 % ） 〉 1D （ 10.34% ）. Total of 107 polymorphic markers were identified in RIL population with a range from 2 to 10 and a mean of 4.65 per each SSR primer. Polymorphism information content（PIC） ranged from 0. 326 to 0. 880 with 1B（0. 709） 〉 1A（0. 534） 〉 1D（0. 498）. Genetic similarity coefficient（GS） ranged from 0.40 to 0.96. According to SSR polymorphic markers, the RIL could be divided into nine subgroups. The ratio of parental genotypes was segregated into 1：1.13 in RIL population. Total of 15 SSR markers showed the significantly segrega- tion distortion（P 〈 0.05） with 65.2% of segregation frequency by Chi-square test,favoring either the marker al- leles of male parent Longjian 19 （7 SSR markers） or female parent Q9086 （8 SSR markers）. Among these segrega- ted SSR markers, six segregated markers was distributed on chromosome 1A,seven markers on 1 B and two on 1D.

关 键 词：小麦 RILs群体 SSR 遗传多样性 偏分离 

分 类 号：S512.3[农业科学—作物学]