坏死梭杆菌QL03株绵羊感染模型的建立及免疫保护性试验  

Study on Development and Immunity of an Infection Model in Sheep Induced with Fusobacterium Necrophorum Isolate QL03 From Dairy Cattle

作　　者：谢晶[1] 廖党金[1] 叶勇刚[1] 林毅[1] 赵新元 涂悒 曹冶[1] 赵素君[1] 李江凌[1] 罗丹丹[1] 于吉峰[1] 

机构地区：[1]四川省畜牧科学研究院,成都610066 [2]凉山半细毛羊原种场

出　　处：《中国草食动物科学》2012年第5期50-53,共4页China Herbivore Science

基　　金：国家支撑计划(2006BAD04A17);四川省支撑计划项目(2010NZ0106);四川省畜牧科学研究院基金;国家奶牛产业技术体系科学家岗位

摘　　要：坏死梭杆菌是奶牛腐蹄病的重要致病菌。用奶牛源坏死梭杆菌QL03分离株以不同浓度菌量感染绵羊蹄部,通过观察绵羊蹄部感染情况、病理变化和动物回归试验,建立了坏死梭杆菌分离株绵羊感染模型。并通过绵羊免疫保护性试验验证了绵羊作为奶牛源坏死梭杆菌QL03分离株动物模型的可行性,为进一步研究坏死梭杆菌有效的疫苗奠定实验基础。Fusobacterium necrophorum is an important pathogen of dairy cattle footrot. In this study, the different dilutions of F. necrophorum Isolate QL03 from dairy cattle were used to inoculate the sheep by subcutaneous injection in the interdigital cleft of the hoof. Through the observation of the infectious development, the pathological changes of the sheep feet and animal regression experiment, the F. necrophorum Isolate QL03 infection model of sheep was established. And it was also suggested that the sheep could be used as the footrot infection model by the immune protection test, which could provide the experimental basis for the Fu- sobacterium necrophorum vaccine research..

关 键 词：坏死梭杆菌 腐蹄病 绵羊 感染模型 免疫 

分 类 号：S851.3[农业科学—预防兽医学]