正交试验优化超声法提取北苍术多糖的工艺研究  

Optimization of ultrasonic extraction process for polysaccharide from Atractylodes chinensis by orthogonal test

作　　者：孟利娜[1] 许静[1] 南楠[1] 段殷[1] 宋晓娇[1] 周晶[1] 

机构地区：[1]天津医科大学药学院天津市临床药物关键技术重点实验室,天津300070

出　　处：《现代药物与临床》2012年第5期457-460,共4页Drugs & Clinic

基　　金：天津市科技攻关项目(06YFGPSH02900)

摘　　要：目的优化北苍术多糖的超声提取工艺。方法利用超声波提取北苍术根茎中的多糖,以提取物得率和多糖提取率为指标,在单因素试验的基础上,采用4个因素(超声时间、料液比、超声温度、乙醇用量)3个水平的L9(34)正交试验优选超声提取工艺条件,并采用苯酚–浓硫酸法测定多糖。结果北苍术多糖的最佳提取工艺条件为:料液比1∶20、提取温度60℃、超声提取40min、浓缩后加入4倍量乙醇进行醇沉。在该实验条件下,苍术水提物得率为23.16%,多糖提取率为11.23%。结论超声法提取苍术中多糖简便快捷,多糖提取率高,为北苍术多糖的开发利用提供了参考。Objective To optimize the extraction technology ofpolysaccharide fromAtractylodes chinensis using ultrasonic extraction method by orthogonal test. Methods Polysaccharide was extracted from A. chinensis by ultrasonic-assisted extraction method. The yield of crude polysaccharide of A. chinensis and extraction rate of polysaccharide were taken as indices. Based on single-factor experiment, the ultrasonic-assisted extraction technology of polysaccharide from A. chinensis was optimized by L9（3^4） orthogonal test. The phenol-sulphuric acid method was applied for the determination ofpolysaccharide. Results The optimal extraction conditions of polysaccharide from A. chinensis were as follows： the solid-liquid ratio was 1 ：20; the extraction temperature was 60 ℃; the extraction time was 40 min and alcohol volume was 4 times of the medicinal materials. Under the conditions, the yield crude polysaccharide and the purity of polysaccharide were 23.16% and 11.23%, respectively. Conclusion Ultrasonic extraction has the advantages of short time, simple operation, and higher extract rate, which could provide the reference for development and utilization ofA. chinensis.

关 键 词：北苍术 多糖 超声提取 正交试验 

分 类 号：R284.2[医药卫生—中药学]