厌氧消化池产甲烷菌的筛选及特性研究  

Research on Screening and Properties of Methanogen from Anaerobic Digester

作　　者：金映虹[1] 黎佳媛[1] 王锐萍[1] 谢秀祯[1] 黄循吟[1] 

机构地区：[1]海南师范大学生命科学学院,海南海口571158

出　　处：《海南师范大学学报（自然科学版）》2012年第3期295-299,共5页Journal of Hainan Normal University（Natural Science）

基　　金：海口市重点科技计划项目(2009030)

摘　　要：从厌氧消化池活性污泥中采集样品,利用特定培养基进行厌氧培养,分离筛选得到2株菌,分别编号为A、B.通过特定荧光反应检测鉴定A、B均为产甲烷菌.利用甲醇液体培养基对A、B两种菌株进行培养,20 d后两株菌均产生甲烷,且通过自制气体收集装置测定其产气量发现,B菌株的产气量高于A菌株.Two strains of Methanogen were isolated from anaerobic digester by anaerobic culture, and identified by the specific fluoroscopy. They were marked as strain A and strain B. A gas collector was designed to collect the methane which was produced by strain A or B. The results showed that strain A and B both could produce gas after about 20 days cultivation with methanol liquid medium, and the gas production of B strain was more than A strain＇ s.

关 键 词：厌氧消化池 产甲烷菌 筛选 

分 类 号：Q939.9[生物学—微生物学]