饲料蛋白水平对不同规格凡纳滨对虾蛋白质表观消化率和消化酶活性的影响  

Effects of dietary protein level on protein apparent digestibility and digestive enzyme activities of Litopenaeus vannamei at different sizes

作　　者：陈义方[1,2] 李卓佳[1] 牛津[1] 王胜 林黑着[1] 陈永青[1] 王芸[1] 黄忠[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部南海渔业资源开发利用重点实验室,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306 [3]广东海大水产与饲料研发中心,广东广州510300

出　　处：《南方水产科学》2012年第5期66-71,共6页South China Fisheries Science

基　　金：现代农业(虾)产业技术体系建设专项资金(CARS-47);公益性行业(农业)科研专项(201103034);广东省科技计划项目(2011A020202007)

摘　　要：研究了7个蛋白质水平(32%、34%、36%、38%、40%、42%和44%)饲料对3个规格(Ⅰ:0.6～4.0 g;Ⅱ:4.0～10.0 g;Ⅲ:10.0～18.0 g)的凡纳滨对虾(Litopenaeus vannamei)蛋白质表观消化率、肝胰脏蛋白酶和淀粉酶活性的影响。规格Ⅰ对虾投喂36%的饲料组具有最大蛋白质表观消化率(85.61%),规格Ⅱ和规格Ⅲ均在40%饲料组达到最大蛋白质表观消化率(分别为84.19%和84.67%)且显著高于其他组。规格Ⅰ40%饲料组具有最高的蛋白酶活力(26.67 U.mg-1)且显著高于44%饲料组,32%饲料组淀粉酶活力显著高于其他组,38%～44%饲料组淀粉酶活性无显著性差异;规格Ⅱ36%饲料组具有最高的蛋白酶活力(25.84 U.mg-1),但各组之间差异不显著,32%～38%饲料组的淀粉酶活力显著高于40%～44%饲料组;规格Ⅲ44%饲料组蛋白酶活力显著高于32%饲料组,42%、44%饲料组淀粉酶活力均显著高于32%饲料组。We investigated the effects of 7 dietary protein levels （32%, 34%, 36%, 38%, 40%, 42% and 44%） on protein apparent digestibility, hepatopancreas protease and amylase activity of Litopenaeus vannamei at 3 sizes （I： 0.6~4.0 g; Ⅱ： 4.0~10.0 g; Ⅲ： 10.0~20.0 g）. The apparent protein digestibility of Size I fed with 36% dietary protein was the highest （85.61%）, and that of Size II and III fed with 40% diet was the highest （84.19% and 84.67%, respectively）, significantly higher than that of the other diets. For SizeⅠ, the protease activity of 40% diet was the highest （26.67 U·mg-1）, significantly higher than that of 44% diet; the amylase activity of 32% diet was significantly higher than that of the other diets, and there was no significant difference among 38%~44% diets. For Size II, the protease activity of 36% diet was the highest （25.84 U·mg-1） but no significant difference was observed among all groups; the amylase activities of 32%~38% diets were significantly higher than those of 40%~44% diets. For Size Ⅲ the protease activity of 44% diet was significantly higher than that of 32% diet; the amylase activity of 42% and 44% diets was significantly higher than that of 32% diet.

关 键 词：凡纳滨对虾 不同规格 肝胰脏 蛋白酶 淀粉酶 蛋白质表观消化率 

分 类 号：S963.71[农业科学—水产养殖]