杭州地区2009甲型H1N1流感重症患儿中人类偏肺病毒感染状况研究  

Study on human metapneumovirus in infants and children with severe 2009 influenza A (H1N1) virus infection in Hangzhou

作　　者：寇宇[1] 于新芬[1] 周银燕[1] 濮小英[1] 郑伟[1] 潘劲草[1] 

机构地区：[1]杭州市疾病预防控制中心,310021

出　　处：《中华危重症医学杂志（电子版）》2012年第4期10-14,共5页Chinese Journal of Critical Care Medicine:Electronic Edition

基　　金：杭州市科技局资助项目(20110833B36)

摘　　要：目的了解杭州地区2009甲型H1N1流感(以下简称甲流)重症患儿中人类偏肺病毒(hMPV)的感染状况。方法采集2009年11月至2010年1月确诊为甲流重症患儿的呼吸道样本79份,用传统逆转录聚合酶链反应(RT-PCR)、荧光定量RT-PCR方法检测hMPV及其他呼吸道病毒。选择hMPV阳性样本PCR扩增产物进行核苷酸测序,将所测序列与GenBank比对分析,并绘制基因进化树。结果 79份甲流病毒阳性样本中,hMPV及其它呼吸道病毒阳性率20.25%(16/79),hMPV阳性PCR扩增产物4份,占甲流重症病例的5.06%(4/79)。其中3份hMPV阳性PCR扩增产物核苷酸序列相似性为99.1%～99.5%,与广东省流行株GD-165,泰国株155N及B1代表株高度相似,并且被GenBank收录。结论杭州地区确诊感染的2009甲型H1N1流感重症病例中存在与hMPV共同感染状况,且hMPV均为B1基因型。Objective To understand the impact of human metapneumovirus virus （hMPV） in infants and children with severe 2009 influenza A （H1N1） infection in Hangzhou. Methods Seventy-nine respiratory specimens from infants and children diagnosed as severe 2009 influenza A （H1N1） infections between November 2009 and January 2010 were collected. The hMPV and other respiratory viruses （including parainfluenza virus, respiratory syncytial virus, adenovirus, coronavirus and rhinovirus） were detected by traditional reverse transcription polymerase chain reaction （RT-PCR） and real-time fluorescent quantitative RT-PCR. The positive PCR products were selected for nueleotide sequencing. The sequence was compared with those in the GenBank and the phylogenetic tree was drawn. Results Among the 79 specimens positive for 2009 influenza A （H1N1）, 16 （20.25%） were detected hMPV and other respiratory viruses, and 4 （5.06%） were detected hMPV-positive both by real-time fluorescent quantitative RT-PCR and traditional RT-PCR. The nucleotide sequences identity were 99.1% ~ 99.5% in 3 positive PCR products. When compared with GD-165, 155N and 99-1 in the GenBank, the 3 positive PCR products showed high similarity, and recorded in the GenBank （genotype B1）. Conclusion The hMPV belonging to genotype B1 in the GenBank could be detected in infants and children with severe 2009 influenza A （H1N1） virus infection in Hangzhou.

关 键 词：变性肺病变 流感病毒A型 H1N1亚型 感染 

分 类 号：R725.1[医药卫生—儿科]