不同干燥加工方法对知母化学成分含量的影响  

The Influence of Different Drying Techniques for the Contents of Effective Ingredients in Rhizoma Anemarrhenae

作　　者：郭晓晔 杨东升[2] 马长华[2] 王文全[2] 黄建梅[2] 尹玉山 

机构地区：[1]北京市宣武中医院药剂科,北京100050 [2]北京中医药大学中药学院,100102 [3]易县太行植物开发有限公司,河北保定074200

出　　处：《中国执业药师》2012年第10期17-20,共4页China Licensed Pharmacist

基　　金：国家科技支撑计划子课题(2006BAI06A15-5)

摘　　要：目的:揭示各种干燥加工方法对知母有效成分含量的影响。方法:分别采用晒干、阴干、烘干、远红外干燥、微波干燥的方法对知母进行干燥加工,以菝葜皂苷元、知母皂苷AⅢ、芒果苷、新芒果苷为指标,通过高效液相色谱法(HPLC),分析各种干燥加工条件下知母中各类指标成分的含量变化情况。其中菝葜皂苷元、知母皂苷AⅢ采用高效液相蒸发光散射检测法(HPLC-ELSD)进行检测,用外标两点法对数方程计算含量,芒果苷、新芒果苷采用高效液相紫外检测法(HPLC-UV)检测。结果:采用烘干、远红外干燥、微波干燥方法加工后,知母菝葜皂苷元、知母皂苷AⅢ、芒果苷含量与晒干、阴干相比均有不同程度上升,新芒果苷含量与晒干、阴干相比有所下降。结论:从有效成分保存方面考虑,不同干燥方法的优劣顺序为微波干燥>远红外干燥>烘干>晒干≈阴干。Objective： To study the influence of different dry techniques for the contents of effective ingredients in rhizoma anemarrhenae. Methods： Fresh rhizoma anemarrhenae was dried with different techniques, such as drying in the sun, drying in the shade, drying in the oven, far-infrared drying and microwave drying, and the contents of the effective ingredients such as sarsasapogenin, anemasaponin A III, neomangiferin and mangiferin were determined by the validated procedure of HPLC. In this study, HPLC-ELSD and two-points external standard methods were employed for the content determination of sarsasapongenin and anemasaponin A III, and HPLC-UV and external standard methods used for the content determination of mangiferin and neomangiferin. Results： Compared with other drying techniques such as drying in the sun or in the shade, higher contents of sarsasapogenin, anemasaponin A m and mangiferin were obtained when the samples processed by drying in the oven, far-infrared drying or microwave drying, while the content ofneomangiferin was lower. Conclusion： For the sake of retaining effective ingredients and taking into consideration of the advantages of different drying techniques, microwave drying, far-infrared drying and drying in the oven can be used preferably other than drying in the sun or in the shade.

关 键 词：知母 干燥加工 菝葜皂苷元 知母皂苷AⅢ 芒果苷 新芒果苷 

分 类 号：R282.710.2[医药卫生—中药学]